A wild-caught stock of greater amberjack Seriola dumerili reared in sea cages in two commercial aquaculture facilities in Greece was administered gonadotropin-releasing hormone agonist (GnRHa), loaded in controlled release implants, and was used in spawning induction experiments throughout the reproductive season. Sperm quality was evaluated using computer-assisted sperm analysis (CASA) before and after GnRHa administration and the extent of the spermiation period was determined. Males were in spermiation throughout the monitoring period from May 30 until July 18, at temperatures between 19 and 24 °C. However, lower sperm motility duration, density, and survival under cold storage were observed from early July onwards. Sperm quality did not recover after the end of spawning induction experiments in tanks and the return of the fish for 14–28 days to the sea cage in mid-July, which could be related to the high temperatures of this period. An improvement trend was observed in the quality of the milt collected on day 7 after a single GnRHa administration, but a significant decrease was observed on day 21 in sperm density, survival under cold storage, and straight line velocity (VSL). On the contrary, a double GnRHa administration spaced 14 days apart maintained the same sperm quality for a longer period of 29 days. Further spermiation enhancement experiments should be conducted in the future to describe in more detail the kinetics of sperm production after GnRHa therapy and its effects on sperm quality.

更大的a鱼Seriola dumerili的野生种群在希腊的两个商业水产养殖设施中的海笼中饲养的动物，接受了促性腺激素释放激素激动剂（GnRHa），装入控释植入物中，并在整个繁殖季节用于产卵诱导实验。在施用GnRHa之前和之后，使用计算机辅助的精子分析（CASA）评估精子质量，并确定精子期的程度。从整个监测期（5月30日至7月18日），男性的精子处于19至24°C的温度下。然而，从7月初开始，在冷藏条件下观察到的精子活动时间，密度和存活率较低。在鱼缸中进行产卵诱导实验结束并在7月中旬将鱼放回海笼中14-28天后，精子质量没有恢复，这可能与这段时期的高温有关。观察到单次GnRHa给药后第7天收集的鱼糜质量有改善趋势，但第21天观察到精子密度，冷藏保存和直线速度（VSL）显着下降。相反，间隔14天的GnRHa双重给药可维持29天的相同精子质量。将来应进行进一步的精子增强实验，以更详细地描述GnRHa治疗后精子产生的动力学及其对精子质量的影响。冷藏条件下的存活率和直线速度（VSL）。相反，间隔14天的GnRHa双重给药可维持29天的相同精子质量。将来应进行进一步的精子增强实验，以更详细地描述GnRHa治疗后精子产生的动力学及其对精子质量的影响。冷藏条件下的存活率和直线速度（VSL）。相反，间隔14天的GnRHa双重给药可维持29天的相同精子质量。将来应进行进一步的精子增强实验，以更详细地描述GnRHa治疗后精子产生的动力学及其对精子质量的影响。