Although silver nanoparticles (AgNP) are among the most studied nanomaterials by virtue of their broad application in many areas, little is known about their overall toxicity to aquatic organisms after their contamination of the water environment. This study aimed to analyze the effect of the exposure (96 h) to different AgNP concentrations on Danio rerio (zebrafish) tissues. AgNP were synthesized and characterized by transmission electron microscopy (TEM), showing spherical AgNP of 30.00 ± 16.80 nm size. The effects of different AgNP concentrations (1, 3, and 5 μg L⁻¹) on brain, muscle, gill, and liver tissues of zebrafish were investigated. The results show a significant decrease in brain and muscle acetylcholinesterase (AChE) activity. Liver and gill catalase (CAT) activity also decreased significantly. At the highest exposure concentration, muscle AChE was more inhibited (37.3%) than brain AChE (26.4%) and gill CAT was more inhibited (67.4%) than liver CAT (51.2%). D. rerio also showed gill morphological changes such as fusion of secondary lamellae, curvature, dilated marginal channel, and epithelial lifting. This study indicates that gill CAT together with morphological studies are potential biomarkers for AgNP.

尽管银纳米颗粒（AgNP）凭借其在许多领域的广泛应用而成为研究最多的纳米材料，但对它们污染水环境后对水生生物的总体毒性知之甚少。这项研究旨在分析暴露于不同AgNP浓度（96 h）对Danio rerio（斑马鱼）组织的影响。合成了AgNP，并用透射电子显微镜（TEM）进行了表征，显示球形AgNP的大小为30.00±16.80 nm。不同AgNP浓度（1、3和5μgL ^-1的影响）在斑马鱼的大脑，肌肉，腮和肝组织上进行了研究。结果表明，大脑和肌肉的乙酰胆碱酯酶（AChE）活性显着下降。肝和g过氧化氢酶（CAT）活性也显着下降。在最高暴露浓度下，肌肉AChE的抑制作用（37.3％）比大脑AChE的抑制作用（26.4％）和g CAT的抑制作用（67.4％）比肝脏CAT（51.2％）更大。D. rerio还显示了g的形态变化，例如继发性薄片融合，曲率，边缘通道扩张和上皮抬升。这项研究表明，g CAT和形态学研究是AgNP的潜在生物标记。