Evernia prunastri (lichen), a novel, eco-friendly, cost-effective, wide availability, safe, renewable and easy collection biosorbent, has been utilized for the removal of Safranin O (SO) dye from an aqueous solution. The biosorption behavior of SO onto the lichen biomass was investigated concerning parameters such as initial SO concentration (10–2000 mg L⁻¹), solution pH (2.0–12.0), lichen biomass dosage (1–20 g L⁻¹), contact time (2–1440 min), temperature (5 °C, 25 °C, and 40 °C), and recovery were investigated. The zeta potential analyses showed that electrostatic attraction existed during the biosorption process between the SO and lichen biomass. The maximum SO biosorption capacity of the lichen biomass was 0.257 mol kg⁻¹ at pH 6.0 and 25 °C. The biosorption energy for SO onto the lichen biomass was found to be E_DR:8.9 kJ mol⁻¹ reveals the biosorption proceeds chemically. The biosorption process follows the pseudo-second order and intra particle diffusion rate kinetics. Thermodynamic studies showed that SO biosorption, by this the lichen biomass is possible, spontaneous, and endothermic.

Evernia prunastri（地衣）是一种新颖，环保，成本效益高，实用性强，安全，可再生且易于收集的生物吸附剂，已用于从水溶液中去除番红O（SO）染料。研究了SO对地衣生物量的生物吸附行为，涉及的参数包括初始SO浓度（10–2000 mg L ^-1），溶液pH（2.0–12.0），地衣生物量（1–20 g L ^-1），接触研究时间（2–1440分钟），温度（5°C，25°C和40°C）和回收率。ζ电势分析表明，SO与地衣生物质之间的生物吸附过程中存在静电吸引。地衣生物质的最大SO吸附量为0.257 mol kg ^-1在pH 6.0和25°C下。发现SO对地衣生物质的生物吸附能为E _DR： 8.9 kJ mol ^-1揭示了化学吸附过程。生物吸附过程遵循伪二级和颗粒内扩散速率动力学。热力学研究表明，SO的生物吸附是可能的，自然的，吸热的是地衣生物质。