Polyhydroxyalkanoates (PHAs) have attracted attention as an environmentally degradable bioplastic which potentially replaces synthetic polymers used in a wide range of industries. One of most promising microorganisms for the production of PHAs is Pseudomonas putida. In this study, we purpose to develop sustainable processes to convert abundant palm oil available in local market to high value PHAs and optimize PHAs production by Pseudomonas putida TISTR 1522 from saponified palm oil. We found that the highest yield of PHAs production (0.95 g/L, 40.15%) was obtained in culture medium supplemented with 1% (w/v) fatty acid salt by P. putida TISTR 1522 after 24-h cultivation. The intracellular PHAs were located in granules inside the cells, which fluoresced bright yellow by staining with Nile red. The physical appearance of intracellular PHAs investigated by transmission electron microscope (TEM) revealed that PHAs accumulate in granules, about 3–10 granules per cell. These granules are white and roundish-shaped with 0.3–0.5-μm diameter. The ¹H NMR spectrum represented the typical characters of medium-chain length-PHAs. This variation of all parameters was successfully demonstrated a good intracellular PHAs accumulation in P. putida TISTR 1522 by fatty acid salt utilization.

聚羟基链烷酸酯（PHA）作为一种可在环境上降解的生物塑料引起了人们的关注，它有可能替代广泛用于各种行业的合成聚合物。产生PHA的最有前途的微生物之一是恶臭假单胞菌。在这项研究中，我们旨在开发可持续的流程，以将本地市场上可用的大量棕榈油转化为高价值的PHA，并优化恶臭假单胞菌TISTR 1522从皂化棕榈油生产的PHAs 。我们发现，在恶臭假单胞菌补充了1％（w / v）脂肪酸盐的培养基中，PHA产量最高（0.95 g / L，40.15％）。培养24小时后的TISTR 1522。细胞内PHA位于细胞内部的颗粒中，通过用尼罗红染色而发出明亮的黄色荧光。通过透射电子显微镜（TEM）研究的细胞内PHA的物理外观表明，PHA堆积在颗粒中，每个细胞约3-10个颗粒。这些颗粒为白色和圆形，直径为0.3-0.5μm。的^1个H NMR谱表示的中等链长的PHA的典型字符。通过脂肪酸盐的利用，成功地证明了所有参数的这种变化在恶臭假单胞菌TISTR 1522中良好的细胞内PHA积累。