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Screening for Ralstonia solanacearum Resistance in Solanum commersonii
American Journal of Potato Research ( IF 1.5 ) Pub Date : 2021-01-06 , DOI: 10.1007/s12230-020-09819-8
Susan A. Otieno , Paul Collins , Joseph Coombs , Caitlyn Allen , David S Douches

Worldwide, potato (Solanum tuberosum L) is rated as one of the most important food crops after wheat, rice and maize. Bacterial wilt, caused by Ralstonia solanacearum (Rs), is one of the most economically important diseases of potato. The purpose of this study was to identify diploid germplasm with resistance to bacterial wilt. The wilting degree was assessed by analyzing the wilt percentage of six Solanum commersonii clones which were artificially inoculated with Rs in a growth chamber. Two Rs strains (CMR15 and UW551) were used to inoculate the clones evaluate resistance. MSEE912–08, a selfed progeny from PI320266 was identified as the most resistant of the clones in this study exhibiting the lowest RAUDPC for wilting and a Wilting Degree rating of 0.4 (less than 25% wilted leaves) 20 days post inoculation when inoculated with the UW551 Rs strain, and 1.3 with the CMR15 strain. The resistance observed was characterized by reduction in wilting over time, rather than lack of disease. The most resistant clone from this study was self-pollinated to create a mapping population to identify single nucleotide polymorphic (SNP) markers associated with Rs resistance.



中文翻译:

Commonersonii茄属中Ralstonia solanacearum抗性的筛选

在世界范围内,马铃薯(马铃薯)被认为是仅次于小麦,水稻和玉米的最重要的粮食作物之一。由青枯雷尔氏菌引起的细菌性枯萎是马铃薯最经济重要的疾病之一。本研究的目的是鉴定对细菌青枯病具有抗性的二倍体种质。通过分析六个茄子的枯萎率来评估枯萎程度在生长室中人工接种Rs的克隆。使用两个Rs菌株(CMR15和UW551)接种克隆以评估抗性。MSEE912–08,来自PI320266的自交后代,在本研究中被鉴定为最具抗性的克隆,表现出最低的RAUDPC枯萎率,接种后20天的枯萎程度等级为0.4(少于25%枯萎的叶片)。 UW551 Rs菌株,而CMR15菌株为1.3。观察到的抗药性的特征是随着时间的推移萎reduction减少,而不是没有疾病。该研究中最抗药性的克隆是自花授粉的,以创建一个定位种群,以鉴定与Rs抗性相关的单核苷酸多态性(SNP)标记。

更新日期:2021-01-06
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