Cancer vaccine is well recognized as a novel but effective way for cancer immunotherapy. Especially, the role of dendritic cells (DCs) in antigen presentation properties is critical for the final performance of cancer vaccine. Herein, a lipid (Li) coated calcium carbonate (CC) vehicle (Li/CC) was employed to load chlorin e6 (Ce6) to serve as a potential in situ vaccine (Li/CC-Ce6) for effective immunotherapy of colorectal cancer. It was suggested that the loaded Ce6 within Li/CCCe6 can be activated under laser irradiation. The photodynamic therapy (PDT) of Ce6 was expected to produce reactive oxygen species (ROS) to cause cell death and expose tumor-associated antigen (TAA). In addition, the produced ROS can mimic the inflammatory responses for the recruitment of DC to initiate strong immune response cascade. Moreover, the recruitment of DC can recognize the exposed TAA to stimulate DC for effective vaccination in situ. Results from in vitro and in vivo assays demonstrated the strong ability of this platform to enhance DC vaccination, resulting in promising growth inhibition of both primary and distant tumors.

中文翻译：

---

脂质涂层和氯霉素e6装载的碳酸钙可用于有效的结直肠癌原位免疫治疗。

癌症疫苗是公认的癌症免疫疗法的一种新颖但有效的方法。特别是，树突状细胞（DCs）在抗原呈递特性中的作用对于癌症疫苗的最终性能至关重要。本文中，使用脂质（Li）涂层的碳酸钙（CC）载体（Li / CC）加载二氢卟酚e6（Ce6）作为原位电势用于大肠癌有效免疫治疗的疫苗（Li / CC-Ce6）。提示可以在激光照射下激活Li / CCCe6中负载的Ce6。预计Ce6的光动力疗法（PDT）会产生活性氧（ROS），从而引起细胞死亡并暴露肿瘤相关抗原（TAA）。另外，产生的ROS可以模拟炎症反应，以募集DC以启动强烈的免疫反应级联。此外，DC的募集可以识别暴露的TAA以刺激DC以进行有效的原位疫苗接种。从结果体外和体内测定证明这个平台的能力强，以增强DC接种，导致有前途的原发性和远处肿瘤的生长抑制。