Ochratoxin is teratogenic, carcinogenic and immunotoxic to humans. It contains ochratoxin in many foods, so the detection of ochratoxin in food is particularly important. In this paper, gold nanoparticles (Au NPs)@g-C₃N₄ composite was synthesized by loading Au NPs on carbon nitride material, and it was immobilized on the surface of glassy carbon electrode by chitosan (Chit) as the substrate of electrochemical aptasensors. An ochratomycin A electrochemical aptasensor was constructed by hybridizing DNA1 and ochratoxin A (OTA) aptamers. The resulting hybrid strands were immobilized on the substrate of glassy carbon electrode. Electrochemical alternating current impedance (EIS) was used to detect the impedance value of the aptasensor when incubating different concentrations of OTA. The impedance value is inversely ratio to the concentration of OTA, achieving quantitative detection of OTA. The aptasensor can detect OTA with a linear range of 0.5-100 ng/mL, the linear correlation coefficient is 0.9506, and the detection limit is 0.167 ng/mL. This aptasensor provides a novel and efficient method for detecting OTA.

中文翻译：

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基于金纳米粒子@g-C₃N₄的O曲霉毒素A传感器的开发。

ch曲毒素对人类具有致畸性，致癌性和免疫毒性。它在许多食品中都含有曲霉毒素，因此食品中曲毒素的检测尤为重要。本文通过在氮化碳材料上负载金纳米颗粒，合成了金纳米颗粒（Au NPs）@g-C₃N₄复合材料，并以壳聚糖（Chit）作为电化学适体传感器的基底固定在玻璃碳电极表面。通过将DNA1和曲霉毒素A（OTA）适体杂交，构建了rat曲霉素A电化学适体传感器。将所得的杂合链固定在玻璃碳电极的基底上。当孵育不同浓度的OTA时，使用电化学交流阻抗（EIS）检测适体传感器的阻抗值。阻抗值与OTA浓度成反比，实现OTA的定量检测。aptasensor可以检测线性范围为0.5-100 ng / mL的OTA，线性相关系数为0.9506，检测极限为0.167 ng / mL。该适体传感器为检测OTA提供了一种新颖而有效的方法。