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Role of mitochondrial complex III/IV in the activation of transcription factor Rst2 in Schizosaccharomyces pombe
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-01-05 , DOI: 10.1111/mmi.14678
Guanglie Jiang 1 , Qiannan Liu 1 , Toshiaki Kato 2 , Hao Miao 1 , Xiang Gao 1 , Kun Liu 1 , Si Chen 1 , Norihiro Sakamoto 2 , Takayoshi Kuno 1, 2 , Yue Fang 1
Affiliation  

Mitochondria play essential roles in eukaryotic cells for glucose metabolism to produce ATP. In Schizosaccharomyces pombe, transcription factor Rst2 can be activated upon glucose deprivation. However, the link between Rst2 and mitochondrial function remains elusive. Here, we monitored Rst2 transcriptional activity in living cells using a Renilla luciferase reporter system, and found that inhibition of mitochondrial complex III/IV caused cells to produce reactive oxygen species (ROS) and nitric oxide (NO), which in turn activated Rst2. Furthermore, Rst2-GFP was observed to translocate from cytoplasm to nucleus upon mitochondrial complex III/IV inhibitors treatment, and deletion of genes associated with complex III/IV resulted in delayed process of Rst2-GFP nuclear exportation under glucose-rich condition. In particular, we found that Rst2 was phosphorylated following the treatment of complex III/IV inhibitors or SNAP. Altogether, our findings suggest that mitochondrial complex III/IV participates in the activation of Rst2 through ROS and NO generation in Schizosaccharomyces pombe.

中文翻译:

线粒体复合物III/IV在粟酒裂殖酵母转录因子Rst2活化中的作用

线粒体在真核细胞中对葡萄糖代谢产生 ATP 起着至关重要的作用。在粟酒裂殖酵母中,转录因子 Rst2 可以在葡萄糖剥夺时被激活。然而,Rst2 和线粒体功能之间的联系仍然难以捉摸。在这里,我们使用Renilla监测活细胞中的 Rst2 转录活性荧光素酶报告系统,并发现线粒体复合物 III/IV 的抑制导致细胞产生活性氧 (ROS) 和一氧化氮 (NO),进而激活 Rst2。此外,观察到 Rst2-GFP 在线粒体复合物 III/IV 抑制剂处理后从细胞质转移到细胞核,并且与复合物 III/IV 相关的基因缺失导致在富含葡萄糖的条件下 Rst2-GFP 核输出的过程延迟。特别是,我们发现 Rst2 在复合 III/IV 抑制剂或 SNAP 处理后被磷酸化。总而言之,我们的研究结果表明线粒体复合物 III/IV 参与了粟酒裂殖酵母中通过 ROS 和 NO 产生的 Rst2 的激活。
更新日期:2021-01-05
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