Using fluorescence quenching of quantum dot nano-particles is a promising technique for determination of chemical species. In most cases these measurements have been done in liquid phase. Immobilization of quantum dot nano-particles on a solid bed offer easier separation, preconcentation, washing the interferences and quick fluorescence measurements of different analyts so that the quantum dots are reusable again. In this study three different approaches for quantum dot immobilization on a glass surface including sol-gel process, thiol-metal affinity and amide bond formation were compared in the time, temperature, atmosphere, solvent availability, pH, efficiency and tunability of attachment yield. The amide bonding was found the best strategy with 24 h reaction time, ambient temperature, air atmosphere, pH=10, no need for anhydrous solvent, highest fluorescence intensity (more than 50% of total intensities) , tunability of attachment with reproducible data (RSD<5.0%), cheap and convenient technique for immobilization of nano-particles on the glass surface.

使用量子点纳米粒子的荧光猝灭是一种确定化学物种的有前途的技术。在大多数情况下，这些测量是在液相中完成的。将量子点纳米颗粒固定在固体床上可以更轻松地进行分离，预浓缩，清洗干扰物并快速测量不同分析物的荧光，从而使量子点可以再次使用。在这项研究中，在时间，温度，气氛，溶剂利用率，pH，效率和附着产率的可调性方面，比较了三种不同的量子点固定在玻璃表面的方法，包括溶胶-凝胶法，硫醇金属亲和力和酰胺键形成。在24小时的反应时间，环境温度，空气气氛，pH = 10，不需要无水溶剂的情况下，发现酰胺键是最佳策略。