Objectives

The relationship between stroke etiology and clot pathology remains controversial.

Materials and Methods

We performed histological analysis of clots retrieved from 52 acute ischemic stroke patients using hematoxylin and eosin staining and immunohistochemistry (CD42b and oxidative/hypoxic stress markers). The correlations between clot composition and the stroke etiological group (i.e., cardioembolic, cryptogenic, or large artery atherosclerosis) were assessed.

Results

Of the 52 clots analyzed, there were no significant differences in histopathologic composition (e.g., white blood cells, red blood cells, fibrin, and platelets) between the 3 etiological groups (P = .92). By contrast, all large artery atherosclerosis clots showed a localized pattern with the oxidative stress marker 4-hydroxyl-2-nonenal (P < .01). From all 52 clots, 4-hydroxyl-2-nonenal expression patterns were localized in 28.8% of clots, diffuse in 57.7% of clots, and no signal in 13.5% of clots.

Conclusions

A localized pattern of 4-hydroxyl-2-nonenal staining may be a novel and effective marker for large artery atherosclerosis (sensitivity 100%, specificity 82%).

中文翻译：

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卒中患者血栓中的4-羟基-2-非局部表达模式与大动脉粥样硬化相关

目标

中风病因与凝块病理之间的关系仍存在争议。

材料和方法

我们使用苏木精和曙红染色和免疫组化（CD42b和氧化/低氧应激标志物）对52例急性缺血性中风患者的血块进行了组织学分析。评估了血块成分与中风病因学组（即心脏栓塞，隐源性或大动脉粥样硬化）之间的相关性。

结果

在分析的52个血凝块中，3个病因组之间的组织病理学组成（例如白细胞，红细胞，纤维蛋白和血小板）没有显着差异（P  = 0.92）。相比之下，所有大动脉粥样硬化凝块均显示具有氧化应激标记物4-hydroxyl-2-nonenal的局部模式（P <.01）。在所有52个凝块中，4-羟基-2-壬烯醛表达模式位于28.8％的凝块中，在57.7％的凝块中扩散，而在13.5％的凝块中没有信号。

结论

4-羟基-2-壬烯染色的局部模式可能是大动脉粥样硬化的一种新型且有效的标记物（敏感性为100％，特异性为82％）。