Industrial hemp has an excellent lead (Pb) tolerance and accumulation capacity. Further enhancing the Pb tolerance and its accumulation in industrial hemp is of great significance for its future application in phytoremediation. In present study, a pot experiment using Pb-contaminated soil was conducted to investigate the accumulation of Pb contents in various organs of industrial hemp and its subcellular distribution and extractable form in the leaves. It was found that under Pb stress, the Pb content in plants increased significantly with an increase in the soil Pb concentration. Lead was mainly concentrated in the roots, followed by stems and leaves, while the lowest Pb contents were found in seeds (<12 mg/kg). The Pb content in roots was 2–7 times higher than in stems, leaves, and fibers, and 6–25 times higher than in seeds. At the subcellular level, Pb in industrial hemp leaves was mainly distributed in the cell wall and chloroplasts, with the Pb content first increasing and then decreasingas the soil Pb concentration increased, reaching a maximum at 4000 and 3000 mg/kg, respectively. Lead was less accumulated in the vacuoles and soluble parts of cells, with the least accumulation occurring in the organelles. There was no nsignificant increase in Pb content in these subcellular locations with the increase in soil Pbconcentration. Treatments with different Pb concentrations affected the extractable form of Pb in hemp leaves. The HCl extractable form of Pb, with low activity, was predominant in the leaves. The proportional content of Pb in each form was different in the various parts of the plant. The Pb content of each form in the leaf was Pb HCl(hydrochloric acid)＞Pb E (ethanol)>Pb W (deionized water)>Pb HAc (acetic acid) >Pb NaCl (sodium chloride), indicating that Pb in industrial hemp mainly exists in the form of insoluble oxalate precipitates, thereby alleviating the toxicity of Pb. The results of this study provide a scientific reference for the use of industrial hemp to remediate Pb polluted soils.

工业大麻具有出色的铅（Pb）耐受性和累积能力。进一步提高对铅的耐受性及其在工业用大麻中的积累对于其在植物修复中的未来应用具有重要意义。在本研究中，进行了盆栽试验，使用了受Pb污染的土壤，以研究工业大麻中各个器官中Pb含量的积累及其在叶片中的亚细胞分布和可提取形式。发现在铅胁迫下，植物中铅的含量随着土壤铅含量的增加而显着增加。铅主要集中在根部，其次是茎和叶，而种子中的铅含量最低（<12 mg / kg）。根中的铅含量比茎，叶和纤维高2-7倍，比种子高6-25倍。在亚细胞水平上，工业大麻叶中的Pb主要分布在细胞壁和叶绿体中，随着土壤Pb浓度的增加，Pb含量先升高然后降低，分别达到4000和3000 mg / kg的最大值。铅在细胞的液泡和可溶性部分中的积累较少，而在细胞器中的积累最少。随着土壤铅含量的增加，这些亚细胞位置的铅含量没有显着增加。不同铅浓度的处理影响了大麻叶中铅的可提取形式。活性较低的Pb的HCl提取形式主要存在于叶片中。在植物的各个部位，每种形式的铅的比例含量都不同。叶片中每种形态的铅含量为：盐酸铅（Pb HCl）＞乙醇（Pb E）>去离子水（Pb）W（去离子水）>醋酸（Pb HAc）>氯化钠（Pb NaCl）>氯化钠（Pb NaCl）。主要以不溶性草酸盐沉淀的形式存在，从而减轻了Pb的毒性。这项研究的结果为工业大麻修复铅污染土壤提供了科学参考。