Inflammation theory has suggested that the pathogenesis of postoperative ileus (POI) involves the steroid receptor coactivator-3 (SRC-3). Therefore, we investigated the role of SRC-3 in the muscles of the small intestine using a mouse POI model. Here, we reported that intestinal manipulation (IM) significantly reduced the extent of phenol red migration in the entire gastrointestinal tract, and the calculated geometric center (GC) value in wild-type (WT) mice at 24 h after surgery was higher than that in the knockout (KO) mice and in the sham-operated control group. The expression of SRC-3 was upregulated in the mouse intestinal muscularis at 24 h after surgical manipulation, and the mRNA and protein levels of inflammatory cytokines were upregulated compared with those in the control group. At 24 h after IM, the number of neutrophils in the experimental group was significantly higher than that in the control group; in the IM group, the number of neutrophils in the SRC-3^-/- mice was markedly higher than that in the WT mice. At 24 h after IM, the myeloperoxidase (MPO) activity in the experimental group was significantly higher than that in the control group. In the IM group, the MPO activity of the SRC-3^-/- mice was markedly higher than that of the WT mice. In summary, proinflammatory cytokines, the number of neutrophils, and the MPO activity were significantly increased in the muscularis of the jejunum and ileum of KO mice after IM compared with those of the WT mice, indicating that SRC-3 might play a protective role in POI.

炎症理论表明术后肠梗阻 (POI) 的发病机制涉及类固醇受体辅激活因子-3 (SRC-3)。因此，我们使用小鼠 POI 模型研究了 SRC-3 在小肠肌肉中的作用。在这里，我们报道了肠道操作（IM）显着降低了整个胃肠道中酚红迁移的程度，并且在手术后24小时野生型（WT）小鼠的计算几何中心（GC）值高于在敲除 (KO) 小鼠和假手术对照组中。手术后24 h小鼠肠肌层SRC-3表达上调，炎性细胞因子mRNA和蛋白水平较对照组上调。在 IM 后 24 小时，实验组中性粒细胞数明显高于对照组；在 IM 组中，SRC-3 ^-/-小鼠明显高于WT小鼠。IM后24小时，实验组髓过氧化物酶（MPO）活性显着高于对照组。在IM组中，SRC-3 ^-/-小鼠的MPO活性明显高于WT小鼠。总之，与 WT 小鼠相比，IM 后 KO 小鼠空肠和回肠肌层的促炎细胞因子、中性粒细胞数量和 MPO 活性显着增加，表明 SRC-3 可能在兴趣点。