The development of maize germplasm with resistance to red flour beetle requires identification of donors of resistant traits and understanding of the genetic mechanisms underlying resistance. As resistance sources are scarce in elite maize lines, teosinte (Zea mays ssp. parviglumis; 2n = 20) following insect bioassay was found to be resistant and therefore used as parent in a cross with flour beetle susceptible but superior maize inbred line DI-103 for development of backcross inbred line (BIL) mapping population with a view to identify genomic regions conferring resistance. Data on four resistance parameters namely, weight loss (WL), number of insect progeny emerged, kernel damage (KD) and flour produced (FP) were collected on 141 BILs. Highly significant differences were found amongst BILs for all the parameters indicating substantial variability in the population for the traits probably contributed by teosinte. Based on cumulative susceptibility index (CSI), 83 BILs were scored as resistant and can be utilized as donor for flour beetle resistance in maize improvement programs. Principal component analysis was conducted and first three principal components contributing 92.36% of the total phenotypic variation were identified. Highly significant positive correlations were found between all trait combinations except for correlation between WL and KD which was positive but non-significant. A total of 18 marker linked genomic regions were found to be significantly associated with red flour beetle resistance. Two major quantitative trait loci (QTLs) were detected on chromosome 1 and 3 for KD and they explained 11.17% and 14.88% of the total phenotypic variation, respectively. Highest number of colocalized QTLs i.e., 3 were detected for KD and FP. These linked markers after further validation can be used in future for indirect selection for resistance to red flour beetle in maize.

对红粉甲虫具有抗性的玉米种质的发展需要鉴定抗性性状的供体并了解抗性的遗传机制。作为抗源稀少在优良玉米线，大刍草（玉蜀黍SSP。parviglumis; 昆虫生物测定后2n = 20）被发现具有抗性，因此可作为易感粉甲虫但优良玉米自交系DI-103的亲本，用于开发回交自交系（BIL）作图种群，以鉴定基因组区域赋予抵抗力。在141个BILs上收集了四个抗性参数的数据，即体重减轻（WL），出现的昆虫后代数量，籽粒损伤（KD）和产生的面粉（FP）。在所有参数的BIL之间发现高度显着差异，表明种群中teosinte可能造成的性状存在很大差异。根据累积敏感性指数（CSI），83个BIL被评为抗药性，可在玉米改良计划中用作抗性的供体。进行了主成分分析，确定了占总表型变异92.36％的前三个主成分。在所有性状组合之间发现高度显着的正相关，但WL和KD之间的相关为正但不显着。发现总共18个标记连锁的基因组区域与红粉甲虫抗性显着相关。在KD的1号和3号染色体上检测到两个主要的定量性状基因座（QTL），它们分别解释了总表型变异的11.17％和14.88％。共定位的QTL数量最多，即KD和FP检测到3个。经过进一步验证的这些连锁标记将来可用于间接选择玉米中的抗红粉甲虫。