We analyzed the prevalence and genetic characteristics of the extended-spectrum β-lactamases (ESBLs)-producing Enterobacterales isolated from adult patients hospitalized in the oncological center in 2019. Out of 9372 patients admitted to the hospital, 1373 had been in various medical facilities during the last year, which was an indication to perform a screening test for ESBL-producing Enterobacterales colonizing their gastrointestinal tract. In eighty-three patients (6.1%), 85 ESBL producers were detected. These isolates included the following: Escherichia coli ( n = 67; 78.8%), Klebsiella pneumoniae ( n = 14; 16.5%), Enterobacter cloacae cplx ( n = 3; 3.5%), and Klebsiella oxytoca ( n = 1; 1.2%). CTX-M-1-like enzymes were the most common ESBLs ( n = 67; 78.8%). Two K. pneumoniae isolates (2/85; 2.4%) additionally produced New Delhi-metallo-β-lactamases (NDM). All isolates, except for K. oxytoca , were typed by pulsed-field gel electrophoresis (PFGE) and demonstrated high genetic diversity. The most prevalent phylogroups of E. coli were B2 group ( n = 30; 44.8%), followed by A group ( n = 25; 37.3%). These observations have motivated us to investigate the link between ESBL-E colonization and infection among patients with solid tumors.

中文翻译：

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肿瘤医院住院患者胃肠道中产 ESBL 肠杆菌的特征

我们分析了 2019 年在肿瘤中心住院的成年患者中分离出的产超广谱 β-内酰胺酶 (ESBLs) 肠杆菌的患病率和遗传特征。在入院的 9372 名患者中，1373 名在住院期间曾在各种医疗机构中去年，这是对在胃肠道中定殖的产 ESBL 肠杆菌进行筛查试验的迹象。在 83 名患者 (6.1%) 中，检测到 85 种 ESBL 生产者。这些分离株包括：大肠杆菌（n = 67；78.8%）、肺炎克雷伯菌（n = 14；16.5%）、阴沟肠杆菌 cplx（n = 3；3.5%）和催产克雷伯菌（n = 1；1.2%） ）。CTX-M-1 样酶是最常见的 ESBL（n = 67；78.8%）。两个肺炎克雷伯菌分离株 (2/85; 2. 4%）另外产生了新德里金属-β-内酰胺酶（NDM）。所有分离株，除 K. oxytoca 外，均通过脉冲场凝胶电泳 (PFGE) 进行分型，并显示出高遗传多样性。大肠杆菌最普遍的系统群是 B2 组 (n = 30; 44.8%)，其次是 A 组 (n = 25; 37.3%)。这些观察结果促使我们研究 ESBL-E 定植与实体瘤患者感染之间的联系。