For the first time, the microalga Porphyridium cruentum was tested for its ability to produce silver nanoparticles. To characterize formed silver nanoparticles UV-vis Spectrometry, Scanning Electron Microscopy, Energy-dispersive analysis of X-rays and X-ray diffraction were used. It was shown that after biomass exposure to silver nitrate solution the extracellular formation of spherical-like nanoparticles took place. Functional groups responsible for metal binding were determined by Fourier-transform infrared spectroscopy. The complex of biochemical tests was used for biomass characterization and assessment of the changes of its main components (proteins, lipids, carbohydrates, and phycobilin) during nanoparticle formation. Obtained data indicate a significant decrease of proteins, carbohydrates, phycobiliproteins, and lipids content as well as antiradical activity of biomass. The obtained results show the necessity of determination of optimal conditions for obtaining Porphyridium cruentum biomass enriched with silver nanoparticles for its further application in the pharmaceuticals industry.

中文翻译：

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与银纳米粒子生物合成相关的微藻Porphyridium cruentum的生化变化

首次测试了微藻 Porphyridium cruentum 生产银纳米粒子的能力。为了表征形成的银纳米颗粒，紫外-可见光谱、扫描电子显微镜、X 射线的能量色散分析和 X 射线衍射被使用。结果表明，在生物质暴露于硝酸银溶液后，发生了球形纳米颗粒的细胞外形成。负责金属结合的官能团由傅立叶变换红外光谱确定。生化测试复合物用于生物量表征和评估其主要成分（蛋白质、脂质、碳水化合物和藻胆素）在纳米颗粒形成过程中的变化。获得的数据表明蛋白质、碳水化合物、藻胆蛋白、和脂质含量以及生物质的抗自由基活性。所得结果表明，有必要确定获得富含银纳米粒子的紫菜生物质的最佳条件，以进一步应用于制药行业。