The golden chanterelle represents one of the commonly found, edible mushrooms that is highly valued in various cuisines. The present study focused on assessing the requirements of Cantharellus cibarius such as pH, temperature, as well as the carbon and nitrogen sources for mycelial growth. Optimization of the growth parameters was carried out by one-factor-at-a-time method. The optimal pH and temperature were determined to be 6.0 and 22.5 °C, respectively. Among the various carbon sources studied, sucrose at a concentration of 2% gave maximum mycelial growth and proved to be the most suitable one. Amongst the nitrogen sources studied, peptone, ammonium sulphate, and sodium nitrate, gave the maximum mycelial growth at an optimized concentration of 0.5%. In the presence of beef extract and yeast extract, a change in colony pigmentation from yellow to dark grey was observed. Finally, the carbon to nitrogen ratio of 2:0.5 proved to be optimal for mycelial growth. This study is the first report on the optimisation of in vitro growth requirements of C. cibarius .

中文翻译：

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实验室条件下鸡油菌菌丝生长需求的研究

金色鸡油菌代表一种常见的可食用蘑菇，在各种美食中都受到高度重视。本研究的重点是评估鸡油菌对菌丝生长的要求，如 pH 值、温度以及碳源和氮源。生长参数的优化是通过一次一个因素的方法进行的。最佳 pH 值和温度分别确定为 6.0 和 22.5 °C。在研究的各种碳源中，浓度为 2% 的蔗糖使菌丝体生长最大，并被证明是最合适的一种。在研究的氮源中，蛋白胨、硫酸铵和硝酸钠在 0.5% 的优化浓度下产生最大菌丝体生长。在牛肉提取物和酵母提取物的存在下，观察到菌落色素沉着从黄色变为深灰色。最后，2:0.5 的碳氮比被证明是最适合菌丝体生长的。本研究是第一份关于优化C. cibarius 体外生长要求的报告。