A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem. The isolate has been characterized based on polyphasic approaches. The isolate exhibit well-developed substrate mycelia along with the presence of cocci- and rod-shaped elements. The organism can grow across a wide range of temperature, salinity, and pH as well as on different carbon sources. Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage). Presence of ketosynthase domain representing polyketide synthases in the isolate provides evidence of its potential ability to produce secondary metabolites. Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria). The DNA G + C content of the isolate has been determined as 72 mol%. The peptidoglycan type was A4α and the whole-cell hydrolysates contained glucose, galactose, and mannose. The polar lipids were represented by diphosphatidylglycerol, one unknown phospholipid and one unknown glycolipid. Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17. Whole-genome sequence indicates the size of genome is ~ 5 Mbp. GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700. The isolate I2 has been proposed as a new species, Myceligenerans indicum sp. nov. The genome sequence has been deposited to GenBank/ENA/DDBJ under the accession number JABBYC000000000.

中文翻译：

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Myceligenerans indicum sp。11 月，从印度孙德尔本斯的红树林沉积物中分离出的放线菌

从印度孙德尔本斯红树林生态系统的潮间带沉积物中分离出一种新的放线菌属菌丝体。基于多相方法对分离物进行了表征。该分离株表现出发育良好的底物菌丝体以及球菌和杆状元件的存在。生物体可以在很宽的温度、盐度和 pH 值范围内以及在不同的碳源上生长。基于 16S rRNA 的系统发育分析表明，该分离株与 Myceligenerans salitolerans XHU 5031 密切相关（99% 同一性；100% 覆盖率）。分离物中代表聚酮合酶的酮合酶域的存在提供了其产生次级代谢物的潜在能力的证据。基于 atpD 和 rpoB 基因序列的多基因系统发育证实它是原小单孢菌科（放线菌门）中的一个新物种。分离物的 DNA G + C 含量已确定为 72 mol%。肽聚糖类型为A4α，全细胞水解物含有葡萄糖、半乳糖和甘露糖。极性脂质由二磷脂酰甘油、一种未知磷脂和一种未知糖脂代表。分离物中存在的主要脂肪酸是反异-C15、异-C15、异-C16 和反异-C17。全基因组序列表明基因组的大小约为 5 Mbp。I2 基因组的GGDC (%)、orthoANIu (%) 和AAI 表明与西里沟菌丝体菌株DSM 15,700 的基因组具有28.9%、77.44% 和0.859 的同一性。分离株 I2 已被提议作为一个新物种，Myceligenerans indicum sp。十一月