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Efficacy of 75% alcohol in pretreatment of the Andersen sampler in trapping maximum airborne microbes
Aerobiologia ( IF 2 ) Pub Date : 2021-01-05 , DOI: 10.1007/s10453-020-09668-2
Yan Zhao 1 , Jingwei Zhang 1 , Shumei Wang 2 , Lingqi Yu 2 , Hao Yu 1 , Yuwen Wang 1 , Lihong Feng 1
Affiliation  

The present study was conducted to evaluate the effects of the pretreatment methods and sampling time on the sampling of airborne bacteria in hospitals. Methods for the pretreatment of Andersen samplers, namely, non-sterilized, 75% ethanol and autoclaving sampled for 5 min, 10 min and 15 min in the general ward and class 1000 clean operating department, respectively, were studied. Statistical analysis was used to compare the differences in sampling results of airborne bacteria under different pretreatment methods, sampling time and environmental conditions. In the first test, the sampling results of the airborne bacteria obtained by pretreatment of the sampler with 75% ethanol and without pre-treatment were not very different, and the sampling results showed a certain declining trend with the extension of the sampling time. In the second test, the pretreatment effect of autoclaving was significantly better than that of 75% ethanol, and the sampling time had no effect on the sampling results. After removing the influencing factors of the environment, the results were consistent with the results of the second test. It was observed that the Andersen samplers should not be pretreated with 75% ethanol before airborne microbes sampling. The pretreatment should be carried out by autoclaving, and the sampling time has little effect on the sampling results.

中文翻译:

75% 酒精预处理安徒生采样器捕获最大空气传播微生物的效果

本研究旨在评估预处理方法和采样时间对医院空气传播细菌采样的影响。研究了安徒生采样器的预处理方法,即在普通病房和1000级洁净手术室分别进行非灭菌、75%乙醇和高压灭菌采样5 min、10 min和15 min。采用统计分析方法比较不同预处理方法、采样时间和环境条件下空气中细菌采样结果的差异。在第一次试验中,采样器用75%乙醇预处理和不预处理得到的空气传播细菌的采样结果差别不大,而且随着采样时间的延长,采样结果呈现出一定的下降趋势。在第二次测试中,高压灭菌的预处理效果明显优于75%乙醇,采样时间对采样结果没有影响。去除环境影响因素后,结果与第二次测试结果一致。据观察,Andersen 采样器在空气传播微生物采样之前不应使用 75% 乙醇进行预处理。预处理应采用高压灭菌法进行,取样时间对取样结果影响不大。据观察,Andersen 采样器在空气传播微生物采样之前不应使用 75% 乙醇进行预处理。预处理应采用高压灭菌法进行,取样时间对取样结果影响不大。据观察,Andersen 采样器在空气传播微生物采样之前不应使用 75% 乙醇进行预处理。预处理应采用高压灭菌法进行,取样时间对取样结果影响不大。
更新日期:2021-01-05
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