Radiation mutagenesis could provide new drought-tolerant lines for selection purposes in sustainable agriculture. Drought tolerance and yield stability are closely related to coping with oxidative stress, which occurs at severe/prolonged water deprivation. In this study, the response of a newly generated winter wheat mutant line M181/1338K to severe drought stress at seedling stage (3–4th leaf) was compared to that of two established varieties—Guinness (drought tolerant) and Farmer (drought sensitive). Oxidative damage and antioxidant status analyses were performed on second leaves of control, stressed (45–46% leaf water deficit) and recovered plants. Genotypes exhibited similar pattern of stress response, comprising proline accumulation, rise in hydrogen peroxide content and oxidative damage to membrane lipids, increase in total antioxidant and antiradical activities, in phenolic and flavonoid content, in ascorbate and glutathione pools, mobilization of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) enzyme isoforms. Farmer responded to severe water stress with the highest levels of oxidative damage to membranes, proline accumulation, and glutathione content, and slower normalization of the studied parameters upon recovery. Guinness presented a better control of oxidative membrane damage and it the highest accumulation of flavonoids under drought. The new mutant line M181/1338K had similarities with Guinness in its response to severe water stress, such as the same proline and glutathione levels. Unlike Guinness, the mutant genotype had more pronounced oxidative damage to membranes, along with higher POX activities, and tended to accumulate less flavonoids under drought, which could be regarded as secondary effects of the induced mutagenesis.

辐射诱变可以为可持续农业的选择提供新的耐旱品系。干旱耐受性和产量稳定性与应对氧化胁迫密切相关，氧化胁迫在严重/长期缺水时会发生。在这项研究中，比较了一个新生成的冬小麦突变体品系M181 / 1338K对苗期（3-4片叶子）的严重干旱胁迫的反应与两个成熟品种-吉尼斯（耐旱）和农民（对干旱敏感）的反应的比较。 。在对照的第二片叶子，胁迫（叶片缺水45-46％）和恢复的植物上进行了氧化损伤和抗氧化状态分析。基因型表现出相似的应激反应模式，包括脯氨酸积累，过氧化氢含量上升和对膜脂质的氧化损伤，增加总抗氧化剂和抗自由基活性，抗坏血酸盐和谷胱甘肽库中的酚和类黄酮含量，超氧化物歧化酶（SOD），过氧化氢酶（CAT）和过氧化物酶（POX）酶同工酶的动员。农民对严重的水分胁迫做出了反应，对膜的氧化损伤，脯氨酸的积累和谷胱甘肽的含量最高，恢复时研究参数的归一化速度较慢。吉尼斯能更好地控制氧化膜损伤，并且在干旱下其类黄酮的积累最高。新突变株M181 / 1338K与吉尼斯在对严重水分胁迫的反应方面具有相似性，例如脯氨酸和谷胱甘肽水平相同。与吉尼斯（Guinness）不同，突变基因型对膜的氧化损伤更为明显，同时POX活性更高，