Biosynthesis methods employing microorganisms have emerged as an eco-friendly, clean, and viable alternative to chemical and physical processes. The present study reports the synthesis of copper oxide nanoparticles (CuONPs) using cell-free culture supernatant of marine Streptomyces sp. MHM38. For the optimized production of CuONPs, the influence of some parameters, such as the concentration of copper sulfate (CuSO₄), reaction time, filtrate to substrate ratio, and pH, was studied. 5 mM of CuSO₄ was optimal for nanoparticle (NP) production. Well-defined CuONP formation occurred after 60 min of incubation when an equal volume of filtrate (cell-free supernatant) to substrate (CuSO₄ solution) was added. UV-visible spectroscopy analysis of CuONPs exhibited a peak at 550 nm, which corresponds to the surface plasmon resonance of CuONPs. Most of the particles were spherical and were 1.72–13.49 nm when measured using a transmission electron microscope. The antimicrobial activity of CuONPs was determined using a well diffusion method against Enterococcus faecalis ATCC 29212, Salmonella typhimurium ATCC 14028, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8939, fungi (Rhizoctonia solani, Fusarium solani, and Aspergillus niger), and yeast (Candida albicans ATCC 10237). The highest antimicrobial activities were recorded against Candida albicans ATCC 10237, whereas Salmonella typhimurium ATCC 14028 and Escherichia coli ATCC 8939 showed the less activity. The biochemical findings of the CuONP groups were significant () with diminished levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total and direct bilirubin, urea, and creatinine compared with the paracetamol group. Nonenzymatic and enzymatic antioxidants of the CuONP groups were significantly elevated () in SOD and GSH levels, and exceptionally low nitric oxide (NO) and malondialdehyde (MAD) levels were found for the paracetamol group. The histopathological examination of the CuONP groups assured the impact of improving CuONPs against paracetamol-induced liver damage.

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链霉菌MHM38生物合成氧化铜纳米粒子及其生物学应用

利用微生物的生物合成方法已经成为化学和物理过程的生态友好，清洁和可行的替代方法。本研究报告使用海洋链霉菌sp。的无细胞培养上清液合成氧化铜纳米颗粒（CuONPs）。MHM38。为了优化CuONP的生产，研究了一些参数的影响，例如硫酸铜（CuSO ₄）的浓度，反应时间，滤液与底物的比率以及pH值。5 mM的CuSO ₄最适合用于生产纳米颗粒（NP）。孵育60分钟后，当等量滤液（无细胞上清液）与底物（CuSO ₄解决方案）。CuONP的紫外-可见光谱分析显示在550 nm处有一个峰，该峰对应于CuONP的表面等离子体激元共振。使用透射电子显微镜测量时，大多数粒子均为球形，并且为1.72–13.49 nm。使用针对一个阱扩散方法测定CuONPs的抗微生物活性粪肠球菌ATCC 29212，鼠伤寒沙门氏菌ATCC 14028，绿脓杆菌ATCC 9027，大肠埃希氏菌ATCC 8939，真菌（纹枯病，茄病镰刀菌，和黑曲霉）和酵母（假丝酵母白色的ATCC 10237）。对白色念珠菌ATCC 10237的抗菌活性最高，而鼠伤寒沙门氏菌ATCC 14028和大肠杆菌ATCC 8939的抗菌活性较低。CuONP组的生化结果显着（）与对乙酰氨基酚组相比，丙氨酸转氨酶（ALT），天冬氨酸转氨酶（AST），碱性磷酸酶（ALP），乳酸脱氢酶（LDH），总胆红素和直接胆红素，尿素和肌酐水平降低。CuONP组的非酶抗氧化剂和酶抗氧化剂均显着升高（）中的SOD和GSH含量，对乙酰氨基酚组的一氧化氮（NO）和丙二醛（MAD）含量异常低。CuONP组的组织病理学检查确保了改善CuONPs对扑热息痛引起的肝损伤的作用。