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Comparative study on formation of protein coronas under three different serum origins
Biointerphases ( IF 2.1 ) Pub Date : 2020-11-13 , DOI: 10.1116/6.0000396
Sun Young Lee 1 , Jin Gyeong Son 1 , Jeong Hee Moon 2 , Sunho Joh 1 , Tae Geol Lee 1
Affiliation  

Nanomaterials form a complex called “protein corona” by contacting with protein-containing biological fluids such as serum when they are exposed to physiological environments. The characteristics of these proteins, which are one of the substantial factors in cellular response, are affected by the interactions between the nanomaterials and the biological systems. Many studies have investigated the biological behaviors of nanomaterials by conducting experiments in vitro and in vivo; however, the origin of the biological materials used is rather inconsistent. This is due to the fact that the composition of the protein coronas may differ depending on the animal origin, not on the composition or size of the nanoparticles. The resulting differences in the composition of the protein coronas can lead to different conclusions. To identify the differences in protein corona formation among sera of different species, we investigated protein coronas of gold and silica nanoparticles in serum obtained from various species. Using comparative proteomic analysis, common proteins adsorbed onto each nanoparticle among the three different sera were identified as highly abundant proteins in the serum. These findings indicate that protein corona formation is dependent on the serum population rather than the size or type of the nanoparticles. Additionally, in the physiological classification of protein coronas, human serum (HS) was found to be rich in apolipoproteins. In conclusion, our data indicate that HS components are different from those of bovine or mouse, indicating that the serum species origin should be carefully considered when selecting a biological fluid.

中文翻译:

三种不同血清来源下蛋白冠形成的比较研究

纳米材料在暴露于生理环境时,会与血清等含蛋白质的生物体液接触,从而形成称为“蛋白质冠”的复合物。这些蛋白质是细胞反应的重要因素之一,其特性受到纳米材料与生物系统之间相互作用的影响。许多研究通过体外体内实验研究了纳米材料的生物学行为。; 然而,所用生物材料的来源却相当不一致。这是因为蛋白质冠的组成可能因动物来源而异,而不是纳米粒子的组成或大小。由此产生的蛋白质电晕组成的差异会导致不同的结论。为了确定不同物种血清中蛋白质电晕形成的差异,我们研究了从不同物种获得的血清中金和二氧化硅纳米颗粒的蛋白质电晕。使用比较蛋白质组学分析,三种不同血清中吸附在每个纳米颗粒上的常见蛋白质被鉴定为血清中的高丰度蛋白质。这些发现表明蛋白质电晕的形成取决于血清群体,而不是纳米颗粒的大小或类型。此外,在蛋白质冠的生理分类中,发现人血清(HS)富含载脂蛋白。总之,我们的数据表明 HS 成分与牛或小鼠的成分不同,表明在选择生物液体时应仔细考虑血清物种来源。
更新日期:2021-01-04
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