The emergence of SARS-CoV-2 highlights the global need for platform technologies to enable the rapid development of diagnostics, vaccines, treatments, and personal protective equipment (PPE). However, many current technologies require the detailed mechanistic knowledge of specific material-virion interactions before they can be employed, for example, to aid in the purification of vaccine components or in the design of a more effective PPE. Here, we show that an adaption of a polymer microarray method for screening bacterial-surface interactions allows for the screening of polymers for desirable material-virion interactions. Nonpathogenic virus-like particles including fluorophores are exposed to the arrays in an aqueous buffer as a simple model of virions carried to the surface in saliva/sputum. Competitive binding of Lassa and Rubella virus-like particles is measured to probe the relative binding properties of a selection of copolymers. This provides the first step in the development of a method for the discovery of novel materials with promise for viral binding, with the next being development of this method to assess absolute viral adsorption and assessment of the attenuation of the activity of live virus, which we propose would be part of a material scale up step carried out in high containment facilities, alongside the use of more complex media to represent biological fluids.

中文翻译：

---

聚合物微阵列可快速识别病毒样颗粒的竞争性吸附剂

SARS-CoV-2的出现凸显了全球对平台技术的需求，以使诊断，疫苗，治疗和个人防护设备（PPE）得以快速发展。然而，许多当前技术在被采用之前，需要特定的材料-病毒体相互作用的详细机械知识，例如，以帮助纯化疫苗成分或设计更有效的PPE。在这里，我们显示了一种适用于筛选细菌-表面相互作用的聚合物微阵列方法，可用于筛选所需材料-病毒体相互作用的聚合物。非致病性病毒样颗粒包括荧光团在内的各种试剂在水性缓冲液中暴露于阵列，作为唾液/痰中携带的病毒粒子的简单模型。测量Lassa和风疹病毒样颗粒的竞争结合力，以探究所选共聚物的相对结合特性。这提供了开发发现具有病毒结合前景的新材料的方法的第一步，接下来是开发该方法以评估绝对病毒吸附和评估活病毒活性的减弱，我们将提议将是在高密闭设施中进行的材料放大步骤的一部分，同时使用更复杂的介质来表示生物流体。