Using high-throughput analysis methods, the present study sought to determine the impact of prenatal high-fat dietary manipulations on isolation-induced ultrasonic vocalization production in both male and female Fmr1mutants on postnatal day 9. Prior to breeding, male FVB/129 Fmr1 wildtype and female Fmr1 heterozygous breeding pairs were assigned to 1 of 3 diet conditions: standard lab chow, omega-3 fatty acid-enriched chow, and a diet controlling for the fat increase. Prenatal exposure to omega-3 fatty acids improved reductions in the number of calls produced by Fmr1heterozygotes females. Moreover, diminished spectral purity in the female Fmr1homozygous mouse was rescued by exposure to both high-fat diets, although these effects were not seen in the male Fmr1knockout. Prenatal dietary fat manipulation also influenced several other aspects of vocalization production, such as the number of calls produced and their fundamental frequency, aside from effects due to loss of Fmr1.Specifically, in males, regardless of genotype, prenatal exposure to high omega-3s increased the average fundamental frequency of calls. These data support the need for future preclinical and clinical work elucidating the full potential of prenatal high-fat diets as a novel therapeutic alternative forFragile X syndrome.
Dev Neurosci

中文翻译：

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产前高脂肪饮食以性别特异性方式挽救 Fmr1 突变小鼠的沟通缺陷

本研究采用高通量分析方法，试图确定产前高脂肪饮食操作对出生后第 9 天雄性和雌性Fmr1突变体隔离诱导的超声波发声产生的影响。在繁殖前，雄性 FVB/129 Fmr1野生型和雌性Fmr1杂合育种对被分配到 3 种饮食条件中的一种：标准实验室饲料、富含 omega-3 脂肪酸的饲料和控制脂肪增加的饮食。产前接触 omega-3 脂肪酸可减少Fmr1杂合子雌性发出的叫声数量。此外，暴露于两种高脂肪饮食可以挽救雌性Fmr1纯合子小鼠的光谱纯度下降，尽管这些影响在雄性Fmr1敲除小鼠中并未观察到。除了 Fmr1 缺失造成的影响之外，产前膳食脂肪控制还影响发声产生的其他几个方面，例如发出的声音数量及其基频。具体来说，在男性中，无论基因型如何，产前接触高 Omega-3 都会增加平均基本叫声频率。这些数据支持未来临床前和临床工作的需要，阐明产前高脂肪饮食作为一种新的治疗替代方案的全部潜力脆性 X 综合征。
开发神经科学