The centrosome, which consists of centrioles and pericentriolar material (PCM), becomes mature and assembles mitotic spindles by increasing the number of microtubules (MTs) emanating from the PCM. Among the molecules involved in centrosome maturation, Cep192 and Aurora A (AurA, also known as AURKA) are primarily responsible for recruitment of -tubulin and MT nucleators, whereas pericentrin (PCNT) is required for PCM organization. However, the role of Cep215 (also known as CDK5RAP2) in centrosome maturation remains elusive. Cep215 possesses binding domains for -tubulin, PCNT and MT motors that transport acentrosomal MTs towards the centrosome. We identify a mitosis-specific centrosome-targeting domain of Cep215 (215N) that interacts with Cep192 and phosphorylated AurA (pAurA). Cep192 is essential for targeting 215N to centrosomes, and centrosomal localization of 215N and pAurA is mutually dependent. Cep215 has a relatively minor role in -tubulin recruitment to the mitotic centrosome. However, it has been shown previously that this protein is important for connecting mitotic centrosomes to spindle poles. Based on the results of rescue experiments using versions of Cep215 with different domain deletions, we conclude that Cep215 plays a role in maintaining the structural integrity of the spindle pole by providing a platform for the molecules involved in centrosome maturation.

由中心粒和中心粒周围物质（PCM）组成的中心体变得成熟，并通过增加从PCM发出的微管（MT）的数量来组装有丝分裂纺锤体。在与中心体成熟有关的分子中，Cep192和Aurora A（AurA，也称为AURKA）主要负责微管蛋白和MT成核剂的募集，而PCM组织则需要percentcentrin（PCNT）。但是，Cep215（也称为CDK5RAP2）在中心体成熟中的作用仍然难以捉摸。Cep215拥有针对微管蛋白，PCNT和MT马达的结合结构域，这些结合结构域将人质MT转运至中心体。我们确定了与Cep192和磷酸化AurA（pAurA）相互作用的Cep215（215N）有丝分裂特定的靶向域。Cep192对于将215N靶向中心体至关重要，215N和pAurA的中心体定位相互依赖。Cep215在微管蛋白募集到有丝分裂中心体中的作用相对较小。但是，以前已经证明该蛋白对于将有丝分裂中心体连接到纺锤极上很重要。基于使用具有不同结构域缺失的Cep215版本进行的抢救实验结果，我们得出结论，Cep215通过为参与中心体成熟的分子提供平台，在维持纺锤体极的结构完整性中发挥作用。