Abstract

The phloroglucinol eugenial C, eugenial D and eugenial E are the main active compounds in Eugenia umbelliflora fruits. This study aims to evaluate the extraction conditions of E. umbelliflora, using ethanol as solvent, focusing on the phloroglucinol and antimicrobial activity. In order to optimize the extraction conditions, ethanol 50, 70 and 90 ^oGL was used as a solvent in the proportions of 1:20 (w/v) of drug:solvent ratio (D:S), stirring (330 rpm) at room temperature during 4 h, monitored by LC-UV and antimicrobial assay. The LC-UV method developed was linear over a concentration range of 3.4–68.0, 5.3–106.0 and 5.0–100.8 μg.mL⁻¹ of eugenial C, eugenial D and eugenial E, having LOQ of 1.68, 1.33 and 0.8 μg.mL⁻¹, respectively. The fruits showed the best herbal raw material and showed the highest phloroglucinol concentration and activity against S. aureus, when extracted with ethanol 90^oGL, during 4 h, at 1:20 of D:S.

摘要

间苯三酚 eugenial C、eugenial D 和 eugenial E 是Eugenia umbelliflora果实中的主要活性成分。本研究旨在评估伞形花藤的提取条件，以乙醇为溶剂，重点考察间苯三酚和抗菌活性。为了优化提取条件，以 1:20 (w/v) 的药物:溶剂比 (D:S) 的比例使用乙醇 50、70 和 90 ^o GL 作为溶剂，以 330 rpm 的速度搅拌室温下 4 小时，通过 LC-UV 和抗菌测定监测。所开发的 LC-UV 方法在 eugenial C、eugenial D 和 eugenial E 的 3.4-68.0、5.3-106.0 和 5.0-100.8 μg.mL ^-1浓度范围内呈线性，LOQ 为 1.68、1.33 和 0.8 μg.mL ^-1， 分别。当用乙醇 90 ^o GL 在 1:20 D:S 提取 4 h 时，果实显示出最好的草药原料，并显示出最高的间苯三酚浓度和对金黄色葡萄球菌的活性。