ABSTRACT

Since the successful creation of DJ-526, a resveratrol-enriched rice cultivar, research has focused on resveratrol production because of its great potential in pharmaceutical applications. However, the utilization of resveratrol in DJ-526 is limited by glycosylation, which converts resveratrol to its glucoside (piceid), in a process driven by glycosyltransferase. The verification of resveratrol-dependent glycosyltransferase activity is an essential strategy for improving resveratrol production in DJ-526 rice. In this study, 27 candidate glycosyltransferases were evaluated in germinated seeds. Among the candidates, only R12 exhibited upregulation related to increased resveratrol and piceid content during seed germination, whereas various effects on the activity of glycosyltransferase were observed by the presence of a bio-elicitor. Yeast extract tended to enhance glycosyltransferase activity by seven candidates, and a specific peak for an unknown compound production was identified. Conversely, chitosan acted as a glycosyltransferase inhibitor. Our results suggested that R12 and R19 are the most relevant candidate resveratrol-dependent glycosyltransferases in DJ-526 seeds during germination and elicitation. Future research should assess the possibility of silencing these candidate genes in an effort to improve resveratrol levels in DJ-526 rice.

摘要

自从成功培育出富含白藜芦醇的水稻品种 DJ-526 以来，由于其在药物应用方面的巨大潜力，研究一直集中在白藜芦醇的生产上。然而，白藜芦醇在 DJ-526 中的利用受到糖基化的限制，糖基化在糖基转移酶驱动的过程中将白藜芦醇转化为其葡萄糖苷 (piceid)。验证白藜芦醇依赖性糖基转移酶活性是提高 DJ-526 水稻白藜芦醇产量的重要策略。在这项研究中，对发芽种子中的 27 种候选糖基转移酶进行了评估。在候选物中，只有 R12 表现出与种子萌发过程中白藜芦醇和 piceid 含量增加相关的上调，而通过生物诱导剂的存在观察到对糖基转移酶活性的各种影响。酵母提取物倾向于通过七种候选物增强糖基转移酶活性，并确定了未知化合物产生的特定峰。相反，壳聚糖充当糖基转移酶抑制剂。我们的结果表明，R12 和 R19 是 DJ-526 种子在萌发和诱导过程中最相关的候选白藜芦醇依赖性糖基转移酶。未来的研究应该评估沉默这些候选基因以提高 DJ-526 水稻中白藜芦醇水平的可能性。我们的结果表明，R12 和 R19 是 DJ-526 种子在萌发和诱导过程中最相关的候选白藜芦醇依赖性糖基转移酶。未来的研究应该评估沉默这些候选基因以提高 DJ-526 水稻中白藜芦醇水平的可能性。我们的结果表明，R12 和 R19 是 DJ-526 种子在萌发和诱导过程中最相关的候选白藜芦醇依赖性糖基转移酶。未来的研究应该评估沉默这些候选基因以提高 DJ-526 水稻中白藜芦醇水平的可能性。