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Rapid and efficient autoclave digestion for the extraction of iodine-129 from urine samples
Journal of Environmental Radioactivity ( IF 2.3 ) Pub Date : 2021-01-04 , DOI: 10.1016/j.jenvrad.2020.106528
Fahad Awwadh Alotaibi 1 , R Jack Cornett 2 , Matthew N Herod 2
Affiliation  

A new method was developed to extract 129I from urine samples and measure it using accelerator mass spectrometry (AMS). The samples were pre-treated in an autoclave with hydrogen peroxide and were then acidified with nitric acid, followed by the precipitation of iodine as silver iodide (AgI) for measurement by AMS. This new procedure is substantially faster than previous methods for the extraction of iodine from urine and results in less chemical waste. The efficiency and reproducibility of this method were evaluated by using 125I as a yield tracer, eventually giving a recovery above 99%. To achieve this, several iterations of the method were required. The method was then successfully applied to measure 129I/127I isotopic ratios and 129I concentrations in 25 human urine samples. The AMS results for 129I in urine ranged 3.3 × 106 atoms/L to 884 × 106 atoms/L and the isotope ratio (129I/127I) in human urine ranged from 7.38 × 10−12 to 3.97 × 10−10 with a median of 1.29 × 10−10. This new method will be useful for investigations into the sources of iodine in the human diet and their relative importance for iodine sufficiency.



中文翻译:

用于从尿样中提取碘 129 的快速高效高压灭菌器消化

开发了一种从尿样中提取129 I 并使用加速器质谱法 (AMS) 对其进行测量的新方法。样品在高压釜中用过氧化氢进行预处理,然后用硝酸酸化,然后将碘沉淀为碘化银 (AgI),用于 AMS 测量。这种新方法比以前从尿液中提取碘的方法要快得多,并且化学废物更少。该方法的效率和重现性通过使用125 I 作为产率示踪剂进行评估,最终回收率超过 99%。为此,需要对该方法进行多次迭代。该方法随后成功应用于测量129 I/ 127 I 同位素比率和25 份人体尿液样本中的129 I 浓度。尿液中129 I的 AMS 结果范围为 3.3 × 10 6原子/L 至 884 × 10 6原子/L,人类尿液中的同位素比(129 I/ 127 I)范围为 7.38 × 10 -12至 3.97 × 10 - 10中位数为 1.29 × 10 -10。这种新方法将有助于研究人类饮食中碘的来源及其对碘充足的相对重要性。

更新日期:2021-01-04
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