Two validated, simple and precise densitometric high-performance thin-layer chromatography (HPTLC) quantification methods were proposed for both qualitative and quantitative estimation of oleuropein in Olea europaea leaves and a pharmaceutical product utilizing normal-phase and reversed-phase silica gel TLC plates. In method I, 10 × 20 cm glass plates coated with 0.2 mm thin layers of normal-phase silica gel 60 containing F₂₅₄ (E-Merck, Germany) and a mixture of ethyl acetate‒methanol‒water (8:1:0.5, V/V) were used as the stationary and the mobile phase, respectively. Method II utilized 10 × 20 cm glass-backed plates supporting 0.2 mm layers of RP-18 silica gel 60 containing F₂₅₄ (E-Merck, Germany) as the stationary phase and green solvents mixture composed of ethanol‒water (5.5:4.5, V/V) as the mobile phase. The two methods resulted in sharp, symmetrical, well-resolved peaks at R_F values of 0.47 ± 0.02 and 0.78 ± 0.03 with linearity ranges 200‒1400 ng/spot (r² = 0.9994) and 200‒1400 ng/spot (r² = 0.9996) for method I and method II, respectively. Spots corresponding to oleuropein were scanned at 200 nm. The two methods complied with the ICH guidelines for validation. Due to simplicity, low cost and short analysis time, the methods can be good alternatives for the quality control of different products containing olive leaves extract or pure oleuropein.

提出了两种经过验证的，简单且精确的高效薄层色谱（HPTLC）定量方法，用于定性和定量估计油橄榄叶和使用正相和反相硅胶TLC板的药品中的橄榄苦苷。在方法I中，在10×20 cm的玻璃板上涂上一层0.2 mm的薄层正相硅胶60，其中含有F ₂₅₄（德国E-Merck）和乙酸乙酯‒甲醇‒水（8：1：0.5，V / V）分别用作固定相和流动相。方法II使用10×20 cm的玻璃背板，支撑着0.2 mm的含F ₂₅₄的RP-18硅胶60层。（E-Merck，德国）作为固定相，由乙醇与水（5.5：4.5，V / V）组成的绿色溶剂混合物作为流动相。两种方法在200 1400 ng / spot（r ²  = 0.9994）和200 1400 ng ng / spot（r ²）的线性范围内，在R _F值分别为0.47±0.02和0.78±0.03时产生清晰，对称，良好的峰。 =方法I和方法II分别为= 0.9996）。在200nm处扫描对应于橄榄苦苷的斑点。两种方法均符合ICH指南进行验证。由于简单，成本低，分析时间短，这些方法可以很好地替代包含橄榄叶提取物或纯橄榄苦苷的不同产品的质量控制。