MicroRNA (miRNA) is an endogenous regulatory small molecule RNA. Growing evidence shows that miRNA plays an important regulatory role in gene expression. Although miRNA is a more intensive regulatory noncoding RNA in recent years, few studies have investigated the regulation of targeting genes involved in bone repair. Meanwhile, as a negative bone regulator, previous studies showed that casein kinase 2-interacting protein 1 (CKIP-1) is closely associated with bone formation and regeneration. However, the gene knockout method may not be suitable for clinical application. Therefore, it was hypothesized that miRNA molecules can inhibit the expression of CKIP-1 and ultimately promote the osteogenesis process. The present study revealed that let-7i-5p plays an important role in the process of fracture healing by inhibiting the expression of CKIP-1. Related research provides a novel gene target for fracture healing.

MicroRNA（miRNA）是一种内源性调控小分子RNA。越来越多的证据表明，miRNA 在基因表达中起着重要的调节作用。尽管近年来 miRNA 是一种更密集的调节性非编码 RNA，但很少有研究调查参与骨修复的靶向基因的调节。同时，作为负性骨调节剂，以往的研究表明酪蛋白激酶2相互作用蛋白1（CKIP-1）与骨形成和再生密切相关。然而，基因敲除方法可能不适合临床应用。因此，假设miRNA分子可以抑制CKIP-1的表达并最终促进成骨过程。本研究表明let-7i-5p通过抑制CKIP-1的表达在骨折愈合过程中发挥重要作用。