Applied Microbiology and Biotechnology ( IF 5 ) Pub Date : 2021-01-04 , DOI: 10.1007/s00253-020-11031-x Paola P. Pereira , Marilina Fernandez , Jonathan Cimadoro , Paola S. González , Gustavo M. Morales , Silvia Goyanes , Elizabeth Agostini
Abstract
The aim of the present study was to obtain an effective vehiculation system in which bacterial agents could maintain viability improving their removal capacity. Herein, we present a novel biohybrid membrane of polymeric nanofibers and free-living bacteria for the simultaneous removal of pollutants. In this system, bacteria are free within the pores between the nanofibers and adsorbed to the surface of the membranes. Association between bacteria and the membranes was performed through a self-formulated medium, and the presence of the bacteria in the polymeric matrix was evidenced through atomic force microscopy (AFM). Biohybrid membranes associated with the remediation agents Bacillus toyonensis SFC 500-1E and Acinetobacter guillouiae SFC 500-1A promoted a reduction of up to 2.5 mg/L of hexavalent chromium and up to 200 mg/L of phenol after 24 h of treatment in synthetic medium containing the contaminants. Similarly, more than 46% of the hexavalent chromium and all of the phenol content were removed after treatment of a tannery effluent with initial concentrations of 7 mg/L of Cr(VI) and 305 mg/L of phenol. Counts of the remediation agents from the membranes were always above 1.107 CFU/g, also in the reutilization assays performed without reinoculation. Biohybrid membranes were hydrolysis-resistant, reusable, and effective in the simultaneous removal of contaminants for more than 5 cycles. Viability of the microorganisms was maintained after long-term storage of the membranes at 4 °C, without the use of microbiological media or the addition of cryoprotectants.
Key points
• Polymeric membranes were effectively associated with the SFC 500-1 remediation consortium
• Biohybrid membranes removed hexavalent chromium and phenol from different matrices
• Removal of contaminants was achieved in many successive cycles without reinoculation
中文翻译:
生物混合膜可有效细菌繁殖并同时去除六价铬(CrVI)和苯酚
摘要
本研究的目的是获得一种有效的增湿系统,其中细菌剂可以维持生存能力,从而提高其去除能力。在这里,我们提出了一种新型的聚合物纳米纤维和自由生活细菌的生物混合膜,用于同时去除污染物。在该系统中,细菌在纳米纤维之间的孔内自由释放并吸附到膜表面。细菌与膜之间的缔合是通过自配制的介质进行的,而聚合物基质中细菌的存在则通过原子力显微镜(AFM)得以证明。与修复剂Toonensis Bacillus toyonensis SFC 500-1E和guillouiae不动杆菌相关的生物混合膜在含有污染物的合成介质中处理24小时后,SFC 500-1A可以减少多达2.5 mg / L的六价铬和多达200 mg / L的苯酚。同样,处理制革厂废水后,初始浓度为7 mg / L的Cr(VI)和305 mg / L的苯酚,去除了46%以上的六价铬和所有苯酚。膜中修复剂的数量始终高于1.10 7CFU / g,也可以在不重新接种的情况下进行的再利用测定中。生物混合膜具有抗水解,可重复使用的特点,并且可以有效地同时去除污染物超过5个循环。膜在4°C下长期保存后,微生物的活力得以维持,而无需使用微生物培养基或添加冷冻保护剂。
关键点
•聚合物膜与SFC 500-1修复联盟有效地联系在一起
•生物混合膜可去除不同基质中的六价铬和苯酚
•在许多连续的循环中去除了污染物,无需重新接种