Background

Human demodecid mites including Demodex folliculorum and Demodex brevis Akbulatova can cause acne, rosacea, epifolliculitis, blepharitis, seborrheic dermatitis, perioral dermatitis, acromastitis and such skin health problems. Artemisia (Composiate) are widely distributed in temperate regions in the northern hemisphere. It has been reported that 17 species of plants in Artemisia used to be mugwort in China. Mugwort volatile oil (MVO) has antibacterial and antiviral effects, can relieve cough and asthma, acts as an expectorant, choleretic and sedative, and promotes circulation and enhances immunity.

Purpose

This research was to observe the effect of MVO on two types of human demodecid mites in vitro.

Methods

The MVO was obtained via the supercritical CO₂ extraction method, and the human demodecid mites were acquired with cellophane tape. MVO had a distinct killing effect on two types of human demodecid mites, Demodex folliculorum and Demodex brevis. The body of the demodecid mites has a classical temporal process, which consists of excitement, contractions, death and transparency. The killing time was lengthened with decreasing concentration, thus showing an evident dependence on concentration.

Results

The experiment showed that 3.125% was the minimum effective concentration of MVO for killing D. brevis, and 6.25% was the minimum effective concentration for killing D. folliculorum; the killing effect of MVO on D. brevis was greater than on D. follilorum.

Conclusion

This result suggests that mugwort, which acts as a traditional Chinese herbal medicine, has a noticeable killing effect on human demodecid mites.

中文翻译：

---

艾叶挥发油对体外人类蠕形螨的影响

背景

包括毛囊蠕形螨和短蠕形螨Akbulatova 在内的人类蠕形螨可引起痤疮、酒渣鼻、表皮毛囊炎、睑缘炎、脂溢性皮炎、口周皮炎、肢端炎等皮肤健康问题。蒿（复合）广泛分布于北半球温带地区。据报道，我国曾有17种艾蒿属植物。艾蒿挥发油（MVO）具有抗菌和抗病毒作用，可止咳平喘，祛痰利胆镇静，促进血液循环，增强免疫力。

目的

本研究旨在观察MVO对两种人类蠕形螨的体外作用。

方法

MVO采用超临界CO ₂萃取法获得，人类蠕形螨采用玻璃纸胶带采集。MVO对毛囊蠕形螨和短蠕形螨两种人类蠕形螨有明显的杀灭作用。蠕形螨的身体有一个经典的时间过程，包括兴奋、收缩、死亡和透明。杀灭时间随着浓度的降低而延长，显示出明显的浓度依赖性。

结果

试验表明，3.125%的MVO杀灭短尾毛虫的最低有效浓度为6.25%，杀灭毛囊毛囊虫的最低有效浓度为6.25% ；MVO对D. brevis的杀灭作用大于对D. follilorum的杀灭作用。

结论

这一结果表明，作为传统中草药的艾蒿对人类蠕形螨具有显着的杀灭作用。