Abstract

Rfam is a database of RNA families where each of the 3444 families is represented by a multiple sequence alignment of known RNA sequences and a covariance model that can be used to search for additional members of the family. Recent developments have involved expert collaborations to improve the quality and coverage of Rfam data, focusing on microRNAs, viral and bacterial RNAs. We have completed the first phase of synchronising microRNA families in Rfam and miRBase, creating 356 new Rfam families and updating 40. We established a procedure for comprehensive annotation of viral RNA families starting with Flavivirus and Coronaviridae RNAs. We have also increased the coverage of bacterial and metagenome-based RNA families from the ZWD database. These developments have enabled a significant growth of the database, with the addition of 759 new families in Rfam 14. To facilitate further community contribution to Rfam, expert users are now able to build and submit new families using the newly developed Rfam Cloud family curation system. New Rfam website features include a new sequence similarity search powered by RNAcentral, as well as search and visualisation of families with pseudoknots. Rfam is freely available at https://rfam.org.

中文翻译：

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Rfam 14：扩大宏基因组学，病毒学和microRNA家族的覆盖范围

摘要

Rfam是RNA家族的数据库，其中3444个家族中的每个家族均由已知RNA序列的多序列比对和可用于搜索该家族其他成员的协方差模型表示。最近的发展涉及专家合作以提高Rfam数据的质量和覆盖范围，重点是microRNA，病毒和细菌RNA。我们已经完成了在Rfam和miRBase中同步microRNA家族的第一阶段，创建了356个新的Rfam家族并更新了40个。我们建立了从黄病毒和冠状病毒科开始全面注释病毒RNA家族的程序RNA。我们还从ZWD数据库中增加了基于细菌和基于基因组的RNA家族的覆盖范围。这些发展使数据库有了显着的增长，在Rfam 14中增加了759个新家庭。为了促进社区对Rfam的进一步贡献，专家用户现在能够使用新开发的Rfam Cloud家庭管理系统建立和提交新家庭。 。Rfam网站的新功能包括由RNAcentral支持的新序列相似性搜索，以及带有假结的家族的搜索和可视化。Rfam可从https://rfam.org免费获得。