Abstract

Splicing is an essential step of RNA processing for multi-exon genes, in which introns are removed from a precursor RNA, thereby producing mature RNAs containing splice junctions. Here, we develope the RJunBase (www.RJunBase.org), a web-accessible database of three types of RNA splice junctions (linear, back-splice, and fusion junctions) that are derived from RNA-seq data of non-cancerous and cancerous tissues. The RJunBase aims to integrate and characterize all RNA splice junctions of both healthy or pathological human cells and tissues. This new database facilitates the visualization of the gene-level splicing pattern and the junction-level expression profile, as well as the demonstration of unannotated and tumor-specific junctions. The first release of RJunBase contains 682 017 linear junctions, 225 949 back-splice junctions and 34 733 fusion junctions across 18 084 non-cancerous and 11 540 cancerous samples. RJunBase can aid researchers in discovering new splicing-associated targets and provide insights into the identification and assessment of potential neoepitopes for cancer treatment.

中文翻译：

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RJunBase：人类正常组织和癌组织中RNA剪接连接的数据库

摘要

剪接是多外显子基因RNA加工的重要步骤，其中将内含子从前体RNA中去除，从而产生含有剪接点的成熟RNA。在这里，我们开发了RJunBase（www.RJunBase.org），这是一个可通过网络访问的数据库，其中包含三种类型的RNA剪接点（线性，反向剪接和融合接点），这些剪接点来自于非癌性和非癌性的RNA序列数据癌组织。RJunBase旨在整合和表征健康或病理性人类细胞和组织的所有RNA剪接连接。这个新的数据库促进了基因水平剪接模式和连接水平表达谱的可视化，以及无注释和肿瘤特异性连接的展示。RJunBase的第一个版本包含682017个线性结，在18 084个非癌性样本和11 540个癌性样本中有225 949个反向剪接连接和34 733个融合连接。RJunBase可以帮助研究人员发现与剪接相关的新靶标，并为鉴定和评估潜在的新表位治疗癌症提供见识。