Discovering nanoscale phenomena to sense biorecognition events introduces new perspectives to exploit nanoscience and nanotechnology for bioanalytical purposes. Here we present Bio Bragg Gratings (BBGs), a novel biosensing approach that consists of diffractive structures of protein bioreceptors patterned on the surface of optical waveguides, and tailored to transduce the magnitude of biorecognition assays into the intensity of single peaks in the reflection spectrum. This work addresses the design, fabrication, and optimization of this system by both theoretical and experimental studies to explore the fundamental physicochemical parameters involved. Functional biomolecular gratings are fabricated by microcontact printing on the surface of tapered optical microfibers, and their structural features were characterized. The transduction principle is experimentally demonstrated, and its quantitative bioanalytical prospects are assessed in a representative immunoassay, based on patterned protein probes and selective IgG targets, in label-free conditions. This biosensing system involves appealing perspectives to avoid unwanted signal contributions from non-specific binding, herein investigated in human serum samples. The work also proves how the optical response of the system can be easily tuned, and it provides insights into the relevance of this feature to conceive multiplexed BBG systems capable to perform multiple label-free biorecognition assays in a single device.

发现纳米现象以感知生物识别事件，为利用纳米科学和纳米技术进行生物分析提供了新的视角。在这里，我们介绍了生物布拉格光栅（BBG），这是一种新颖的生物传感方法，它由在光波导表面上构图的蛋白质生物受体的衍射结构组成，并且经过专门设计，可以将生物识别测定的幅度转换为反射光谱中单个峰的强度。这项工作通过理论和实验研究来探讨该系统的设计，制造和优化，以探索所涉及的基本物理化学参数。通过在锥形光学微纤维的表面上进行微接触印刷来制造功能性生物分子光栅，并对其结构特征进行了表征。实验证明了转导原理，并且在无标记条件下，基于模式化蛋白质探针和选择性IgG靶标，可以在代表性的免疫分析中评估其定量生物分析前景。该生物传感系统涉及吸引人的观点，以避免由非特异性结合引起的不希望的信号贡献，本文在人血清样品中进行了研究。这项工作还证明了如何轻松调节系统的光学响应，并为该功能的相关性提供了见识，以设想能够在单个设备中执行多个无标记生物识别测定的多路BBG系统。在无标签的条件下。该生物传感系统涉及吸引人的观点，以避免由非特异性结合引起的不希望的信号贡献，本文在人血清样品中进行了研究。这项工作还证明了如何轻松调节系统的光学响应，并为该功能的相关性提供了见识，以设想能够在单个设备中执行多个无标记生物识别测定的多路BBG系统。在无标签的条件下。该生物传感系统涉及吸引人的观点，以避免由非特异性结合引起的不希望的信号贡献，本文在人血清样品中进行了研究。这项工作还证明了如何轻松调节系统的光学响应，并为该功能的相关性提供了见识，以设想能够在单个设备中执行多个无标记生物识别测定的多路BBG系统。