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Description of Cohnella zeiphila sp. nov., a bacterium isolated from maize callus cultures
Antonie van Leeuwenhoek ( IF 2.6 ) Pub Date : 2021-01-03 , DOI: 10.1007/s10482-020-01495-2
Eric T Johnson 1 , Patrick F Dowd 1 , Christopher D Skory 2 , Christopher A Dunlap 1
Affiliation  

A Gram-stain positive, aerobic, motile, rod-shaped bacterium designated as strain CBP-2801T was isolated as a contaminant from a culture containing maize callus in Peoria, Illinois, United States. The strain is unique relative to other Cohnella species due to its slow growth and reduced number of sole carbon sources. Phylogenetic analysis using 16S rRNA indicated that strain CBP-2801T is a Cohnella bacterium and showed the highest similarity to Cohnella xylanilytica (96.8%). Genome-based phylogeny and genomic comparisons based on average nucleotide identity confirmed the strain to be a novel species of Cohnella. Growth occurs at 15–45 °C (optimum 40 °C), pH 5–7 (optimum pH 6) and with 0–1% NaCl. The predominant fatty acids are anteiso-15:0 and 18:1 ω6c. Genome mining for secondary metabolites identified a putative biosynthetic cluster that encodes for a novel lasso peptide. In addition, this study contributes five new genome assemblies of type strains of Cohnella species, a genus with less than 30% of the type strains sequenced. The DNA G + C content is 58.7 mol %. Based on the phenotypic, phylogenetic and biochemical data strain CBP-2801T represents a novel species, for which the name Cohnella zeiphila sp. nov. is proposed. The type strain is CBP-2801T (= DSM 111598 = ATCC TSD-230).



中文翻译:

Cohnella zeiphila sp. 的描述。11 月,一种从玉米愈伤组织培养物中分离的细菌

从美国伊利诺伊州皮奥里亚的含有玉米愈伤组织的培养物中分离出一种革兰氏染色阳性、需氧、活动、杆状细菌,命名为 CBP-2801 T菌株,作为污染物。由于其生长缓慢和唯一碳源数量减少,该菌株相对于其他Cohnella物种而言是独一无二的。使用 16S rRNA 进行的系统发育分析表明,菌株 CBP-2801 T是一种Cohnella细菌,与Cohnella xylanilytica 的相似性最高(96.8%)。基于基因组的系统发育和基于平均核苷酸同一性的基因组比较证实该菌株是Cohnella的新物种. 在 15–45 °C(最佳 40 °C)、pH 5–7(最佳 pH 6)和 0–1% NaCl 条件下生长。主要的脂肪酸是 anteiso-15:0 和 18:1 ω6 c。次生代谢物的基因组挖掘确定了一个假定的生物合成簇,该簇编码一种新型套索肽。此外,这项研究贡献了五个新的Cohnella物种类型菌株的基因组组装,该属的类型菌株测序不到 30%。DNA G + C 含量为 58.7 mol%。基于表型、系统发育和生化数据,菌株 CBP-2801 T代表了一个新物种,其名称为Cohnella zeiphila sp。十一月 被提议。类型菌株是 CBP-2801 T (= DSM 111598 = ATCC TSD-230)。

更新日期:2021-01-03
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