Abstract

Genome-wide mutagenesis induced by N-ethyl-N-nitrosourea (ENU) results in efficient introduction of mutations into the genome of mammalian spermatogonial cells. This feature is being used to generate animals with phenotypes associated with defects in various functional systems. In particular, using this methodology, it was possible to identify molecular mechanisms of immune responses, identify genes that regulate the development of various organs, etc. This minireview covers genetic studies of immunological and inflammatory reactions in mice using ENU mutagenesis, which led to important findings concerning the regulation of critical signaling pathways of innate and adaptive immunity.

中文翻译：

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小鼠全基因组诱变：寻找调节免疫应答和炎症的基因

摘要

由N-乙基-N-亚硝基脲（ENU）诱导的全基因组诱变可将突变有效地引入哺乳动物精原细胞的基因组中。此功能已用于生成具有与各种功能系统中的缺陷相关的表型的动物。尤其是，使用这种方法，可以识别免疫应答的分子机制，识别调节各个器官发育的基因等。这项小型综述涵盖了使用ENU诱变对小鼠免疫和炎性反应进行基因研究的重要内容。关于先天和适应性免疫的关键信号通路调控的发现。