Abstract

To study the genetic differentiation and phylogeny of the breeds and populations of reindeer (Rangifer tarandus L.), a test system based on multiplex PCR analysis of 16 highly polymorphic STR markers (Rt6, BMS1788, Rt30, Rt1, Rt9, FCB193, Rt7, BMS745, С143, Rt24, OheQ, С217, С32, NVHRT16, T40 and C276) was developed. Using this test system, 397 animals from 11 samples were studied. Four of these samples belonged to the wild forms of reindeer, while the rest of the samples represented the Nenets, Even, and Evenk breeds reared in different climatic zones of the Russian Federation. For 16 microsatellite loci, 204 alleles were identified, some of which could be considered breed-specific. It was demonstrated that more than 70% of the unique allele pool was concentrated in wild populations of reindeer. Statistical analysis of microsatellite data showed that the developed test system was an effective tool for identifying domesticated and wild forms of reindeer, testing the breed assignment, and determining the migration flows of a species over the vast territory of its distribution.

中文翻译：

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利用新的STR标记对驯鹿（Rangifer tarandus）及其野生祖先品种的遗传评价。

摘要

研究驯鹿（Rangifer tarandus）品种和种群的遗传分化和系统发育L.），基于16种高度多态STR标记（Rt6，BMS1788，Rt30，Rt1，Rt9，FCB193，Rt7，BMS745，С143，Rt24，OheQ，С217，С32，NVHRT16，T40和C276的多重PCR分析的测试系统） 发展了。使用该测试系统，研究了来自11个样品的397只动物。这些样品中有四个属于野生形式的驯鹿，而其余样品代表在俄罗斯联邦不同气候区饲养的涅涅茨人，偶人和埃文克犬。对于16个微卫星基因座，鉴定了204个等位基因，其中一些可以被认为是特定品种的。结果表明，超过70％的独特等位基因库集中在野生驯鹿种群中。对微卫星数据的统计分析表明，开发的测试系统是识别驯养和野生驯鹿形式的有效工具，