Porcine circovirus type 2 (PCV2) is the primary agent responsible for porcine circovirus-associated diseases (PCVADs), which is acknowledged as one of the most economically important diseases for the swine industry worldwide. Currently, the development of PCV2 vaccine against PCVADs and for other applications require large amounts of viral particles. The low propagation rate of PCV2 in vitro limits vaccine production. Previous studies showed that a cell line transfected with the porcine interleukin (IL)-2 gene gave higher PCV2 yield in vitro. However, transient transfection may become less effective and unstable after serial generations. In this work, we constructed a PK15 cell line with stable expression of porcine IL2 by lentivirus transfection. The results demonstrated that the transgenic cell line stably expressed IL2 protein significantly enhanced PCV2 replication. Thus, the transgenic PK15 cell line could be a promising cell line for vaccine production.

猪圆环病毒 2 型 (PCV2) 是导致猪圆环病毒相关疾病 (PCVAD) 的主要病原体，PCVAD 被公认为全球养猪​​业最重要的经济疾病之一。目前，针对 PCVAD 和其他应用的 PCV2 疫苗的开发需要大量病毒颗粒。PCV2 的体外低繁殖率限制了疫苗的生产。先前的研究表明，用猪白细胞介素 (IL)-2 基因转染的细胞系在体外可产生更高的 PCV2 产量。然而，瞬时转染在连续世代后可能变得不那么有效和不稳定。在这项工作中，我们通过慢病毒转染构建了稳定表达猪 IL2 的 PK15 细胞系。结果表明，稳定表达IL2蛋白的转基因细胞系显着增强了PCV2的复制。因此，转基因 PK15 细胞系可能是一种很有前途的疫苗生产细胞系。