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hRNAi-mediated knock-down of Sphenophorus levis V-ATPase E in transgenic sugarcane (Saccharum spp interspecific hybrid) affects the insect growth and survival
Plant Cell Reports ( IF 6.2 ) Pub Date : 2021-01-03 , DOI: 10.1007/s00299-020-02646-5
Chakravarthi Mohan 1 , Priscila Yumi Tanaka Shibao 1 , Fernando Fonseca Pereira de Paula 1 , Danyelle Toyama 1 , Marcos Antonio Sanches Vieira 2 , Antonio Figueira 3 , Danielle Scotton 3 , Andrea Soares-Costa 1 , Flavio Henrique-Silva 1
Affiliation  

Key message

Transgenic sugarcane expressing V-ATPase subunit E dsRNA affects growth and survival of Sphenophorus levis.

Abstract

Plants being sessile organisms are constantly confronted with several biotic and abiotic stresses. Sugarcane (Saccharum spp) is a major tropical crop widely cultivated for its sugar and other by-products. In Brazil, sugarcane plantations account for significant production losses due to Sphenophorus levis (sugarcane weevil) infestations. With the existing control measures being less effective, there arises a necessity for advanced strategies. Our bioassay injection experiments with V-ATPase E dsRNA in S. levis larvae showed significant mortality and reduction in transcription levels. Furthermore, we down-regulated the V-ATPase E gene of S. levis in transgenic sugarcane using an RNAi approach. The resultant RNAi transgenic lines exhibited reduction in larval growth and survival, without compromising plant performance under controlled environment. Our results illustrate that RNAi-mediated down-regulation of key genes is a promising approach in imparting resistance to sugarcane weevil.



中文翻译:

hRNAi介导的转基因甘蔗(Saccharum spp种间杂种)中Sphenophorus levis V-ATPase E的敲低影响昆虫的生长和存活

关键信息

表达V-ATPase亚基E dsRNA 的转基因甘蔗影响Sphenophorus levis的生长和存活。

抽象的

作为固着生物的植物不断面临多种生物和非生物胁迫。甘蔗 ( Saccharum spp) 是一种主要的热带作物,因其糖和其他副产品而广泛种植。在巴西,甘蔗种植园因Sphenophorus levis(甘蔗象鼻虫)侵染而造成重大生产损失。由于现有的控制措施效果不佳,因此需要采取先进的策略。我们在S. levis幼虫中使用V-ATPase E dsRNA 进行的生物测定注射实验显示出显着的死亡率和转录水平的降低。此外,我们下调了S. levis的V-ATPase E基因在转基因甘蔗中使用 RNAi 方法。由此产生的 RNAi 转基因品系表现出幼虫生长和存活的减少,而不会影响受控环境下的植物性能。我们的研究结果表明,RNAi 介导的关键基因下调是一种很有前途的方法,可以增强对甘蔗象鼻虫的抗性。

更新日期:2021-01-03
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