In this study, the production and physicochemical characterization of exopolysaccharide (EPS) produced by Lysinibacillus fusiformis KMNTT-10 were investigated. Also, in vitro assays were conducted to investigate the biotechnological functional activities of EPS, including flocculation, emulsification, and antioxidant activity. Results showed that the maximum EPS produced by strain KMNTT-10 was 4.19 g/l after 120 h. The monosaccharides present in EPS were xylose, rhamnose, arabinose, galactose, and glucose in varying combinations. Characterization of EPS by FTIR and XRD analysis revealed the presence of hydroxyl, amino, and carboxyl functional groups and further, the EPS is an amorphous nature. SEM envisaged a highly porous web-like structure with an irregular surface in the EPS morphology. Further, the EPS had good flocculation activity with a maximum of 89.66% and also had potent emulsifying activity towards hydrocarbons with 1% EPS concentration. The EPS showed good antioxidant activity by potent scavenging actions on DPPH, ABTS, and nitric oxide free radicals. Overall results demonstrated that EPS of KMNTT-10 could be used in various industrial applications due to its excellent flocculation, emulsification potential, and good antioxidant activity.

在这项研究中，生产和理化特征的胞外多糖（fusformus fusiformis）研究了KMNTT-10。另外，进行了体外测定以研究EPS的生物技术功能活性，包括絮凝，乳化和抗氧化活性。结果表明，菌株KMNTT-10产生的最大EPS在120 h后为4.19 g / l。EPS中存在的单糖是木糖，鼠李糖，阿拉伯糖，半乳糖和葡萄糖，且存在不同的组合。通过FTIR和XRD分析对EPS进行表征，发现存在羟基，氨基和羧基官能团，此外，EPS是无定形的。SEM设想了EPS形态中具有不规则表面的高度多孔的网状结构。另外，EPS具有良好的絮凝活性，最大为89.66％，并且对于具有1％EPS浓度的烃也具有有效的乳化活性。EPS通过对DPPH，ABTS和一氧化氮自由基的有效清除作用而显示出良好的抗氧化活性。总体结果表明，由于KMNTT-10具有优异的絮凝，乳化潜力和良好的抗氧化活性，因此可用于各种工业应用。