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Development and validation of an analysis method for pesticide residues by gas chromatography–tandem mass spectrometry in Daikenchuto
Journal of Natural Medicines ( IF 3.3 ) Pub Date : 2021-01-03 , DOI: 10.1007/s11418-020-01473-y
Hirokazu Saegusa , Hiroshi Nomura , Masaki Takao , Takashi Hamaguchi , Masaru Yoshida , Yuzo Kodama

Daikenchuto (DKT) is one of the most widely used “Kampo” in Japan as a representative of herbal medicine. Because DKT is made from a natural product like food, it requires the management of pesticides; therefore, an analysis of residual pesticides in Kampo is required. The World Health Organization (WHO) indicates that pesticide residue analysis by the U.S. Pharmacopeia (USP) is required. USP defines 107 compounds containing organochlorine pesticides and organophosphorus pesticides and their metabolites, which have a high residual risk. Accordingly, to guarantee the safety of herbal medicines according to global standards is a very important issue. In this study, we developed an analytical method for 91 compounds, which are listed in USP, using DKT as the subject. The method could extract pesticides from DKT with acetone, elute pesticides with acetonitrile using a SepPak C18 column (5 g) and with ethyl acetate using a DSC-NH2 column (2 g), and perform simultaneous analyses by gas chromatography–tandem mass spectrometry (GC–MS/MS). This method, which could quantify 88 compounds, was validated according to USP. A pesticide residue analysis method that meets USP requirements enables the analysis of pesticide residues with a high residue risk and contributes to improving the safety of “Kampo” and other herbal medicines.



中文翻译:

大根中部气相色谱-串联质谱法分析农药残留的方法的建立与验证

Daikenchuto(DKT)是日本代表草药的最广泛使用的“ Kampo”之一。由于DKT是由食品等天然产品制成,因此需要对农药进行管理;因此,需要对甘榜中的残留农药进行分析。世界卫生组织(WHO)指出,需要通过美国药典(USP)进行农药残留分析。USP定义了107种含有有机氯农药和有机磷农药及其代谢物的化合物,这些化合物具有较高的残留风险。因此,根据全球标准保证草药的安全性是非常重要的问题。在这项研究中,我们以DKT为研究对象,开发了USP中列出的91种化合物的分析方法。该方法可以用丙酮从DKT中提取农药,2柱(2 g),并通过气相色谱-串联质谱(GC-MS / MS)进行同时分析。此方法可定量88种化合物,已根据USP进行了验证。符合USP要求的农药残留分析方法可以分析残留风险较高的农药残留,并有助于提高“ Kampo”和其他草药的安全性。

更新日期:2021-01-03
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