Drug-resistant infectious diseases have increased in recent years. Accordingly, plenty of researches are exploring novel approaches to overcome this problem. In this era, antimicrobial peptides have been identified as potential antibacterial agents. The Modified CM11 (mCM11) was designed with the C-terminal amidation and substitution of lysine with arginine. The designed peptide was synthesized by the solid-phase method and Rink amide p-methyl-benzhydryl amine resin. The synthesized peptide was evaluated using Mass Spectrometry (MS), High-Performance Liquid Chromatography (HPLC), and Circular Dichroism (CD). Finally, the antibacterial, cytotoxic, and apoptotic effect of the mCM11 peptide was investigated. The new peptide indicated a beta-sheet structure with a molecular weight of 1527.50 D and purity of 96%. The peptide exerted a potent antimicrobial activity against gram-negative and positive bacteria. The minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) ranged from 16 to 64 µg/ml, and 16 to 128 µg/ml, respectively. The IC₅₀ of mCM11 was 16 µg/ml and its cytotoxicity in SH-SY5Y cell line revealed a dose-dependent manner. Also, apoptosis analysis of eukaryotic cells revealed a decline in late apoptosis and necrosis in comparison with untreated cells. The mCM11 indicated a considerable antibacterial effect against a wide range of pathogenic bacterial strains. Further, it did not represent any late apoptotic and necrosis impact on the eukaryotic cell line. All of these findings may confirm the potential role of this new peptide as an effective therapeutic agent.

近年来，耐药性传染病有所增加。因此，大量的研究正在探索克服该问题的新颖方法。在这个时代，抗菌肽已被鉴定为潜在的抗菌剂。修饰的CM11（mCM11）设计为C端酰胺化并用精氨酸取代赖氨酸。通过固相法和Rink酰胺对甲基-苯甲基胺树脂合成了设计的肽。使用质谱（MS），高效液相色谱（HPLC）和圆二色谱（CD）评估合成的肽。最后，研究了mCM11肽的抗菌，细胞毒性和凋亡作用。新的肽显示出β-折叠结构，分子量为1527.50 D，纯度为96％。该肽对革兰氏阴性和阳性细菌具有有效的抗菌活性。最小抑菌浓度（MIC）和最小细菌浓度（MBC）分别为16至64 µg / ml和16至128 µg / ml。集成电路₅₀ mCM11的为16微克/毫升及在SH-SY5Y细胞系的细胞毒性揭示了剂量依赖的方式。而且，真核细胞的凋亡分析显示与未处理的细胞相比，晚期凋亡和坏死的下降。mCM11对多种致病细菌菌株显示出显着的抗菌作用。此外，它不代表对真核细胞系的任何晚期凋亡和坏死影响。所有这些发现可能证实了这种新肽作为有效治疗剂的潜在作用。