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Molecular characterization and phylogenetic analysis of Wx genes of four diploid species of the Triticeae genera Eremopyrum , Psathyrostachys , and Henrardia
Genetic Resources and Crop Evolution ( IF 2 ) Pub Date : 2021-01-03 , DOI: 10.1007/s10722-020-01069-1
Shoufen Dai , Junru Li , Jiangwei Huang , Zhi Zhai , Wanjun Yang , Qianyu Liu , Yuanyuan Zuo , Zhongping Song , Jian Li , Gang Liu , Guoyue Chen , Yuming Wei , Zehong Yan

In wheat endosperm, the granule-bound starch synthase or the Waxy protein is the sole enzyme for amylose synthesis and thus determines the amylose content and ratio of amylose/amylopectin. The gene encoding this enzyme has been named as Waxy (Wx) gene. In this study, eight novel Wx alleles from four diploid species belonging to the three Triticeae genera, namely Eremopyrum, Psathyrostachys, and Henrardia have been isolated and characterized. The genetic diversity of these genes was evaluated at the molecular sequence level, and the nucleotides of these Wx genes were also compared with their homologs from wheat and its relatives. The Wx alleles of P. juncea (Fischer) Nevski, E. triticeum (Gaertn.) Nevski, E. bonaepartis (Sprengel) Nevsk, and H. persica (Boiss.) C. E. Hubbard showed obvious differences from the homologous alleles known from wheat and Aegilops as well as Taeniatherum in terms of nucleotide composition, intron length, isoelectric point, and molecular mass. Phylogenetic analysis suggested that the Wx alleles of these four species distantly related to wheat were more closely related to those of Hordeum and Secale than the other species. These Wx alleles are novel candidates with potential application in wheat breeding for modified starch properties.



中文翻译:

小麦(Triticeae)属(Eemopypyrum),沙竹(Psathyrostachys)和黑Hen(Henrardia)的四个二倍体物种Wx基因的分子鉴定和系统发育分析。

在小麦胚乳中,颗粒结合的淀粉合酶或Waxy蛋白是直链淀粉合成的唯一酶,因此决定了直链淀粉的含量和直链淀粉/支链淀粉的比例。编码该酶的基因被称为WaxyWx)基因。在这项研究中,八个新颖的蜡质从属于三个小麦属,即4二倍体种等位基因新麦草Henrardia已经分离和鉴定。在分子序列水平上评估了这些基因的遗传多样性,并将这些Wx基因的核苷酸与小麦及其近亲的同源物进行了比较。该蜡质的等位基因麦草(费歇尔)麦草,E. triticeum(冰草)麦草,E. bonaepartis(史普格尔)Nevsk,和H.桃(羌活。)CE哈伯德表明从小麦和已知的同源等位基因明显的差异节节如以及Taeniatherum的核苷酸组成,内含子长度,等电点和分子量。系统发育分析表明,这四个与小麦密切相关的物种的Wx等位基因与其他大麦大麦西卡莱Wx等位基因关系更密切。这些Wx 等位基因是新颖的候选物,具有潜在的小麦改良淀粉特性的育种应用。

更新日期:2021-01-03
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