Wild relatives represent an important genetic pool for crop improvement. In wheat, a popular strategy for the introduction of alien genes is by manipulation of the Ph1 gene, to induce recombination between homoeologous chromosomes. However, the frequency of such homoeologous recombination is low and requires screening of large progeny samples. Consequently, the efficiency of selection of homoeologous recombinants is important. In this study, we developed two kompetitive allele-specific PCR (KASP) markers specific for chromosome arms 6VS of Haynaldia villosa L. and 6AS of wheat, Triticum aestivum L., one close to the terminal regions and the other near the centromeres. Of the 121 progeny from plants heterozygous for the 6VS·6AL translocation and homozygous for ph1b screened by the two KASP markers, three (2.5%) have shown an exchange of markers, and their recombinant nature was confirmed by in situ DNA hybridization. A recombinants chromosome with a small 6VS fragment carrying the powdery mildew resistance gene ( Pm21 ) would be useful in wheat breeding. The two KASP markers tested here can be used for efficient detection of recombinants between 6AS and 6VS.

中文翻译：

---

KASP标记检测Haynaldia villosa 6VS和小麦6AS之间的亚染色体臂易位

野生近缘种代表了作物改良的重要遗传库。在小麦中，引入外来基因的一种流行策略是通过操纵 Ph1 基因来诱导同源染色体之间的重组。然而，这种同源重组的频率很低，需要筛选大量的后代样本。因此，选择同源重组体的效率很重要。在这项研究中，我们开发了两种竞争性等位基因特异性 PCR (KASP) 标记，这些标记特异于 Haynaldia villosa L. 的染色体臂 6VS 和小麦的 6AS，一个靠近末端区域，另一个靠近着丝粒。在由两个 KASP 标记筛选的 6VS·6AL 易位杂合子和 ph1b 纯合子植物的 121 个后代中，三个 (2.5%) 显示了标记交换，并通过原位 DNA 杂交证实了它们的重组性质。带有携带白粉病抗性基因（Pm21）的6VS小片段的重组染色体可用于小麦育种。这里测试的两个 KASP 标记可用于有效检测 6AS 和 6VS 之间的重组体。