The chitinases are gaining much attention based on their role in the defense against pathogen attacks and harmful insects. The partially chitinase produced by Bacillus licheniformis strain J24 exhibited a large antifungal spectrum, and the highest activity was obtained toward Fusarium species in vitro on PDA and in vivo on corn seeds. The chitinase was inducible by the presence of autoclaved Fusarium conidia in the medium culture and it was active at 70 °C and pH 7 and not affected by the tested chemical agents EDTA and SDS. The nucleotide and amino acid sequences encoding chitinase showed the close phylogenetic relation with chitinase from Bacillus paralicheniformis species. Based on the analysis of the putative domain active, the described chitinase from strain J24 was belonging to the GH family-18 and the novelty of its structure was revealed. Here the combination of functional and structural antifungal extremely chitinase proves its importance in biotechnology area.

中文翻译：

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耐盐地衣芽孢杆菌产生的抗真菌几丁质酶的潜力和表征

几丁质酶因其在防御病原体攻击和有害昆虫方面的作用而备受关注。地衣芽孢杆菌 J24 菌株产生的部分几丁质酶表现出较大的抗真菌谱，在体外对镰刀菌属物种的 PDA 和体内对玉米种子的活性最高。几丁质酶可通过培养基培养物中高压灭菌的镰刀菌分生孢子的存在而诱导，并且在 70 °C 和 pH 7 时具有活性，并且不受测试的化学试剂 EDTA 和 SDS 的影响。编码几丁质酶的核苷酸和氨基酸序列与来自副地衣芽孢杆菌的几丁质酶具有密切的系统发育关系。基于对假定域活性的分析，来自菌株 J24 的所述几丁质酶属于 GH 家族 18，并揭示了其结构的新颖性。