The tule elk (Cervus canadensis nannodes) is endemic to California, USA, where it experienced an extreme genetic bottleneck in the 1870s. Two other subspecies of elk co-occur in California, Roosevelt (C. c. roosevelti) and Rocky Mountain (C. c. nelsoni) elk. These subspecies potentially interbreed in contact zones. For monitoring and genetic management of fragmented populations, markers are needed to differentiate both individuals and subspecies. Using Illumina HiSeq sequencing of 12 individuals, including four from each subspecies, we developed a suite of 96 SNP markers for a Fluidigm platform. This marker set enabled differentiation of individuals and subspecies with high confidence and will facilitate genetic monitoring of elk throughout their range.

北美麋鹿（Cervus canadensis nannodes）是美国加利福尼亚州的特有种，在1870年代经历了极端的遗传瓶颈。麋鹿的两个亚种其他共同出现在加利福尼亚州，罗斯福（Ç。℃。roosevelti）和洛矶山（C.℃。nelsoni）麋鹿。这些亚种可能在接触区杂交。为了监测零散的种群，需要进行标记以区分个体和亚种。我们使用12个个体的Illumina HiSeq测序，包括每个亚种的四个个体，我们为Fluidigm平台开发了一套96个SNP标记。该标记集可高度自信地区分个体和亚种，并将有助于在整个范围内对麋鹿进行遗传监测。