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Genome-Wide Identification of Copper Stress-Regulated and Novel MicroRNAs in Mulberry Leaf
Biochemical Genetics ( IF 2.4 ) Pub Date : 2021-01-03 , DOI: 10.1007/s10528-020-10021-y
Qiuxia Du 1 , Peng Guo 1 , Yisu Shi 1 , Jian Zhang 1 , Danyan Zheng 1 , Yang Li 1 , Adolf Acheampong 1 , Ping Wu 1 , Qiang Lin 2 , Weiguo Zhao 1
Affiliation  

Copper (Cu) is an essential trace element for plant growth and development. It is widely involved in respiration, photosynthesis, pollen formation, and other biological processes. Therefore, low or excessive copper causes damage to plants. Mulberry is an essential perennial economic tree. At present, research on the abiotic stress responses in mulberry is mainly focused on the identification of resistant germplasm resources and cloning of resistant genes. In contrast, studies on the resistance function of microRNAs and the regulatory gene responses to stress are rare. In this study, small RNA libraries (control and copper stressed) were constructed from mulberry leaf RNA. High-throughput sequencing and screening were employed, a total of 65 known miRNAs and 78 predicted novel mature miRNAs were identified, among which 40 miRNAs were differentially expressed under copper stress. Subsequently, expression patterns were verified for 14 miRNAs by real-time fluorescence quantitative PCR (qPCR). The target genes of miRNAs were validated by 5′ RLM-RACE. Our results provide the bases for further study on the molecular mechanism of copper stress regulation in mulberry.



中文翻译:

全基因组鉴定的铜叶中的铜胁迫调控和新的microRNA。

铜(Cu)是植物生长和发育必不可少的微量元素。它广泛参与呼吸,光合作用,花粉形成和其他生物过程。因此,铜含量低或过多都会对植物造成损害。桑树是必不可少的多年生经济树。目前,对桑树非生物胁迫的研究主要集中在抗性种质资源的鉴定和抗性基因的克隆上。相反,关于微RNA的抗性功能和调控基因对压力的反应的研究很少。在这项研究中,从桑叶RNA中构建了小的RNA文库(对照和铜胁迫)。采用高通量测序和筛选,共鉴定出65种已知的miRNA和78种预测的新型成熟miRNA,其中40个miRNA在铜胁迫下差异表达。随后,通过实时荧光定量PCR(qPCR)验证了14种miRNA的表达模式。miRNA的靶基因通过5'RLM-RACE验证。我们的研究结果为进一步研究桑树铜胁迫调控的分子机理提供了依据。

更新日期:2021-01-03
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