Green synthesis of nanoparticles (NPs) had shown the positive effects on various parameters of human health. However, this can vary depending on plant category, NPs nature, doses of green synthesized NPs and type of cell or tissue taken for experiment. The aim of this study is to evaluate the effects of Allium cepa (A. cepa)-mediated zinc oxide ZnO NPs on male reproductive tissues and aberrations in sperm structure of albino mice. The green-synthesized NPs were synthesized with A. cepa and further characterized through ultraviolet–visible spectroscopy, scanning electron microscopy (SEM), zeta potential and FTIR analysis. Various doses (75 mg, 150 mg, 225 mg and 300 mg/kg) were given orally to adult albino mice by keeping them in various groups for 28 days. Epididymis and testis were removed for assessment of spermatogenesis and histopathological analysis of testicular tissues was performed. The synthesized NPs showed absorption at 330 nm and size ranging from 50 to 90 nm through SEM. Dose-dependent significant decrease (P < 0.05) in sperm calculation, increased in sperm abnormalities and sperm motility were observed by the A. cepa mediated ZnO NPs compared to control group. Similarly, testicular tissues showed vacuolization and detachment in seminiferous tubules and degenerative sperm cells in experimental groups provided with synthesized ZnO NPs. The study concluded that ZnO NPs adversely effect on epididymal sperm parameters and testicular tissues of male albino mice.

纳米粒子（NPs）的绿色合成已显示出对人类健康各个参数的积极影响。但是，这可能会有所不同，具体取决于植物类别，NP的性质，绿色合成NP的剂量以及用于实验的细胞或组织的类型。这项研究的目的是评估葱属cepa（A. cepa）介导的氧化锌ZnO NPs对白化小鼠雄性生殖组织和精子结构畸变的影响。绿色合成的NPs与A. cepa合成并通过紫外-可见光谱，扫描电子显微镜（SEM），ζ电位和FTIR分析进一步表征。通过将成年的白化病小鼠分成不同的组放置28天，将其口服（75 mg，150 mg，225 mg和300 mg / kg）。除去附睾和睾丸以评估精子发生，并进行睾丸组织的组织病理学分析。合成的NP通过SEM显示出在330nm处的吸收和尺寸为50至90nm的范围。洋葱曲霉观察到精子计算中剂量依赖性显着降低（P  <0.05），精子异常和精子运动性增加。介导的ZnO NPs与对照组相比。同样，在提供合成的ZnO NP的实验组中，睾丸组织的曲细精管和变性精子细胞出现空泡化和脱离。研究得出结论，ZnO NP对雄性白化病小鼠的附睾精子参数和睾丸组织有不利影响。