Abstract

The aim of this research work was to study the chemical characterisation, antioxidant and cytotoxic activity of ethanolic extracts of four commercial algae species Arame, Kombu, Hijiki and Wakame. The highest scavenging activity has been observed in Arame extract. Antioxidant potential of all extracts was in correlation with total phenol content (Arame extract: 319.15 ± 0.56 mg GAE/g d.w) and it was not in correlation with total carotenoids content (Wakame: 75.15 ± 0.20 mg/g). Polyphenols were quantified using LC-MS/MS technique. Baicalein and amentoflavone were identified in higher amount in relation to other phenols. Intracellular antioxidant activity and cytotoxicity of algae extracts were evaluated on the human prostate cancer cell line PC3. Although presented biomolecules in the extracts have demonstrated in vitro antioxidant activity, they did not show a significant effect on PC3 cells. However, this study opens up broad perspective for the further comprehensive investigation of these, commercial, seaweed’s biopotential.

摘要

这项研究工作的目的是研究四种商业藻类 Arame、Kombu、Hijiki 和 Wakame 的乙醇提取物的化学特征、抗氧化和细胞毒活性。在 Arame 提取物中观察到最高的清除活性。所有提取物的抗氧化潜力与总酚含量相关（Arame 提取物：319.15 ± 0.56 mg GAE/g dw），与总类胡萝卜素含量无关（裙带菜：75.15 ± 0.20 mg/g）。使用 LC-MS/MS 技术对多酚进行定量。与其他酚类相比，黄芩素和橙皮黄酮的含量更高。在人前列腺癌细胞系 PC3 上评估了藻类提取物的细胞内抗氧化活性和细胞毒性。尽管提取物中存在的生物分子已在体外证明抗氧化活性，它们对 PC3 细胞没有显着影响。然而，这项研究为进一步全面调查这些商业海藻的生物潜力开辟了广阔的前景。