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Novel polymorphisms in Stearoyl‐CoA Desaturase (SCD) and Fatty Acid Desaturase 2 (FADS2) in channel catfish (Ictalurus punctatus, Rafinesque, 1818)
Journal of Applied Ichthyology ( IF 0.9 ) Pub Date : 2021-01-01 , DOI: 10.1111/jai.14155
Oliva Mendoza‐Pacheco 1 , Gaspar Manuel Parra‐Bracamonte 1 , Xochitl Fabiola De la Rosa‐Reyna 1 , Ana María Sifuentes‐Rincón 1 , Isidro Otoniel Montelongo‐Alfaro 2 , Ana Laura Lara‐Rivera 3
Affiliation  

The Channel catfish is one of the most important freshwater fish species in North America. Conventionally their selection for genetic improvement is focused in growth related traits but not on quality traits as flavor and fatty acid deposition. The aim of this study was the sequencing of Stearoyl‐CoA Desaturase (SCD) and Fatty Acid Desaturase 2 (FADS2) genes, two of main genes in the biosynthesis of long chain polyunsaturated fatty acids, for novel variation discovering as a preliminary of marker assisted broader assessment. In SCD gene, two novel synonym polymorphisms (Exon 1 and 3) and a non‐synonymous Serine coding polymorphism in exon 3, were found. In FADS2, two synonymous polymorphisms (Exon 1 and 3) and multiple polymorphisms in non‐coding regions were discovered . Further association analysis of these novel variations could reveal punctual effect on fatty acid deposition traits and their utility for marker assisted selection purposes.

中文翻译:

channel鱼硬脂酰-CoA去饱和酶(SCD)和脂肪酸去饱和酶2(FADS2)的新型多态性(Ictalurus punctatus,Rafinesque,1818)

海峡cat鱼是北美最重要的淡水鱼类之一。常规上,它们用于遗传改良的选择集中在与生长相关的性状上,而不是在诸如风味和脂肪酸沉积的品质性状上。这项研究的目的是对长链多不饱和脂肪酸生物合成中的两个主要基因,硬脂酰辅酶A去饱和酶(SCD)和脂肪酸去饱和酶2(FADS2)基因进行测序,以发现新的变异作为标记辅助的初步研究。更广泛的评估。在SCD基因中,发现了两个新的同义词多态性(外显子1和3)和外显子3中的非同义词丝氨酸编码多态性。在FADS2中,发现了两个同义多态性(第1外显子3)和非编码区中的多个多态性。
更新日期:2021-01-22
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